Non-leaching, highly biocompatible nanocellulose surfaces that efficiently resist fouling by bacteria in an artificial dermis model
| Autor: | Tom Coenye, Nina Forsman, Declan C. Mullen, Per E. J. Saris, Blair F. Johnston, Susanne Stehl, Vânia M. Moreira, Leena-Sisko Johansson, Michael Chrubasik, Xing Wan, Monika Österberg, Frits van Charante, Luis M. Bimbo, Jari Yli-Kauhaluoma, Leena Keurulainen, Ralf Zimmermann, Carsten Werner, Ghada S. Hassan, Aruna S. Prakash |
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| Přispěvatelé: | Division of Pharmaceutical Chemistry and Technology, Drug Research Program, Department of Microbiology, Antimicrobials, probiotics and fermented food, Pharmaceutical Design and Discovery group, Jari Yli-Kauhaluoma / Principal Investigator, University of Helsinki, Department of Bioproducts and Biosystems, University of Strathclyde, Leibniz-Institut für Polymerforschung Dresden, Ghent University, Bioproduct Chemistry, Aalto-yliopisto, Aalto University |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
education
116 Chemical sciences Biomedical Engineering cellulose nanofibril Nanotechnology 02 engineering and technology 010402 general chemistry medicine.disease_cause 01 natural sciences biofilm RS Nanocellulose Biomaterials medicine NANOPARTICLES surface STAPHYLOCOCCUS-AUREUS 11832 Microbiology and virology Fouling biology Chemistry DERIVATIVES Biochemistry (medical) NANOFIBRILLATED CELLULOSE Biofilm General Chemistry MASS-SPECTROMETRY ELECTROKINETICS 021001 nanoscience & nanotechnology Antimicrobial biology.organism_classification 0104 chemical sciences 3. Good health dehydroabietic Leaching (chemistry) Staphylococcus aureus DISCOVERY ACID Surface modification FUNCTIONALIZATION antimicrobial dehydroabietic acid 0210 nano-technology ANTIBIOTICS Bacteria |
| Zdroj: | ACS Applied Bio Materials ACS APPLIED BIO MATERIALS |
| ISSN: | 2576-6422 |
| Popis: | Bacterial biofilm infections incur massive costs on healthcare systems worldwide. Particularly worrisome are the infections associated with pressure ulcers and prosthetic, plastic, and reconstructive surgeries, where staphylococci are the major biofilm-forming pathogens. Non-leaching antimicrobial surfaces offer great promise for the design of bioactive coatings to be used in medical devices. However, the vast majority are cationic, which brings about undesirable toxicity. To circumvent this issue, we have developed antimicrobial nanocellulose films by direct functionalization of the surface with dehydroabietic acid derivatives. Our conceptually unique design generates non-leaching anionic surfaces that reduce the number of viable staphylococci in suspension, including drug-resistant Staphylococcus aureus, by an impressive 4-5 log units, upon contact. Moreover, the films clearly prevent bacterial colonization of the surface in a model mimicking the physiological environment in chronic wounds. Their activity is not hampered by high protein content, and they nurture fibroblast growth at the surface without causing significant hemolysis. In this work, we have generated nanocellulose films with indisputable antimicrobial activity demonstrated using state-of-the-art models that best depict an "in vivo scenario". Our approach is to use fully renewable polymers and find suitable alternatives to silver and cationic antimicrobials. |
| Databáze: | OpenAIRE |
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