The 3D8 single chain variable fragment protein suppresses Newcastle disease virus transmission in transgenic chickens

Autor:	Gunsup Lee, Jung-Hoon Kwon, Hwi-Cheul Lee, Shanmugam Sureshkumar, Hyeon Yang, Jin-Yong Noh, Seong-Su Yuk, Sung June Byun, Jae-Seok Woo, Sun Keun Jung, Jeom Sun Kim, Chang-Seon Song, Keon Bong Oh, Hoonsung Choi
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Male animal structures 040301 veterinary sciences Transgene Newcastle Disease viruses Newcastle disease virus (NDV) chicken Newcastle disease virus Virulence chemical and pharmacologic phenomena Newcastle disease Virus 0403 veterinary science Animals Genetically Modified 03 medical and health sciences Single-chain variable fragment Animals Poultry Diseases 030304 developmental biology transgenic 0303 health sciences Nuclease lcsh:Veterinary medicine General Veterinary biology 04 agricultural and veterinary sciences General Medicine respiratory system biology.organism_classification 3D8 ScFv Virology Specific Pathogen-Free Organisms embryonic structures biology.protein Nucleic acid Immunohistochemistry lcsh:SF600-1100 Female Chickens Single-Chain Antibodies Research Article
Zdroj:	BMC Veterinary Research, Vol 16, Iss 1, Pp 1-6 (2020) BMC Veterinary Research
ISSN:	1746-6148
DOI:	10.1186/s12917-020-02462-9
Popis:	Background The 3D8 single chain variable fragment (scFv) is a mini-antibody sequence that exhibits independent nuclease activity against all types of nucleic acids. In this research, crossing a 3D8 scFv G1 transgenic rooster with wild-type hens produced 3D8 scFv G2 transgenic chickens to evaluate suppression of viral transmission. Result The transgenic chickens were identified using genomic PCR and immunohistochemistry. To evaluate Newcastle disease virus (NDV) protection conferred by 3D8 scFv expression, transgenic, non-transgenic, and specific pathogen-free (SPF) chickens were challenged with virulent NDV by direct injection or aerosol exposure. The three groups of chickens showed no significant differences (p Conclusions Our finding suggest that the 3D8 scFv could be a useful tool to prevent chickens from spreading NDV and control virus transmission.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f1a51b9f62096c49d351e4c7434c9ae http://link.springer.com/article/10.1186/s12917-020-02462-9 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.