New insights from Opisthorchis felineus genome: update on genomics of the epidemiologically important liver flukes
| Autor: | Simon Ming-Yuen Lee, Dmitry A. Afonnikov, Svetlana V. Tsygankova, Kirill Ustyantsev, He Zhang, Viatcheslav A. Mordvinov, Mikhail A. Genaev, Victor V. Solovyev, Alexander Mazur, Eugenia S. Boulygina, An Xiao, Xin Liu, Konstantin V. Gunbin, Huanming Yang, Ekaterina Khrameeva, Xun Xu, Egor Prokhortchouk, Alexander Blinov, Nikolay Chekanov, Nikita I. Ershov, Mariya Y. Pakharukova, Guangyi Fan, Konstantin G. Skryabin |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
lcsh:QH426-470 lcsh:Biotechnology Population Cyprinidae Sequence Homology Trans-splicing Biology 01 natural sciences Opisthorchiasis 03 medical and health sciences lcsh:TP248.13-248.65 Cricetinae parasitic diseases Genetics medicine Animals Metacercariae Opisthorchis felineus Microintrons Amino Acid Sequence education 030304 developmental biology Comparative genomics 0303 health sciences education.field_of_study Genome Helminth Clonorchis sinensis Genome Opisthorchiidae Opisthorchis Genomics Helminth Proteins Liver fluke medicine.disease biology.organism_classification lcsh:Genetics Liver flukes Clonorchiasis Transcriptome 010606 plant biology & botany Biotechnology Research Article |
| Zdroj: | BMC Genomics BMC Genomics, Vol 20, Iss 1, Pp 1-22 (2019) |
| ISSN: | 1471-2164 |
| Popis: | Background The three epidemiologically important Opisthorchiidae liver flukes Opisthorchis felineus, O. viverrini, and Clonorchis sinensis, are believed to harbour similar potencies to provoke hepatobiliary diseases in their definitive hosts, although their populations have substantially different ecogeographical aspects including habitat, preferred hosts, population structure. Lack of O. felineus genomic data is an obstacle to the development of comparative molecular biological approaches necessary to obtain new knowledge about the biology of Opisthorchiidae trematodes, to identify essential pathways linked to parasite-host interaction, to predict genes that contribute to liver fluke pathogenesis and for the effective prevention and control of the disease. Results Here we present the first draft genome assembly of O. felineus and its gene repertoire accompanied by a comparative analysis with that of O. viverrini and Clonorchis sinensis. We observed both noticeably high heterozygosity of the sequenced individual and substantial genetic diversity in a pooled sample. This indicates that potency of O. felineus population for rapid adaptive response to control and preventive measures of opisthorchiasis is higher than in O. viverrini and C. sinensis. We also have found that all three species are characterized by more intensive involvement of trans-splicing in RNA processing compared to other trematodes. Conclusion All revealed peculiarities of structural organization of genomes are of extreme importance for a proper description of genes and their products in these parasitic species. This should be taken into account both in academic and applied research of epidemiologically important liver flukes. Further comparative genomics studies of liver flukes and non-carcinogenic flatworms allow for generation of well-grounded hypotheses on the mechanisms underlying development of cholangiocarcinoma associated with opisthorchiasis and clonorchiasis as well as species-specific mechanisms of these diseases. Electronic supplementary material The online version of this article (10.1186/s12864-019-5752-8) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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