Spanish wastewater reveals the current spread of Monkeypox virus

Autor: Inés Girón-Guzmán, Azahara Díaz-Reolid, Pilar Truchado, Albert Carcereny, David García-Pedemonte, Bruno Hernáez, Albert Bosch, Rosa María Pintó, Susana Guix, Ana Allende, Antonio Alcamí, Alba Pérez-Cataluña, Gloria Sánchez
Přispěvatelé: European Commission, Consejo Superior de Investigaciones Científicas (España), Fundación Séneca, Ministerio de Sanidad (España), Ministerio de Transición Ecológica (España)
Rok vydání: 2023
Předmět:
Zdroj: Water Research. 231:119621
ISSN: 0043-1354
DOI: 10.1016/j.watres.2023.119621
Popis: Besides nasopharyngeal swabs, monkeypox virus (MPXV) DNA has been detected in a variety of samples such as saliva, semen, urine and fecal samples. Using the environmental surveillance network previously developed in Spain for the routine wastewater surveillance of SARS-CoV-2 (VATar COVID-19), we have analyzed the presence of MPXV DNA in wastewater from different areas of Spain. Samples (n = 312) from 24 different wastewater treatment plants were obtained between May 9 (week 19 of 2022) and August 4 (week 31 of 2022). Following concentration of viral particles by a validated aluminum adsorption-precipitation method, a qPCR procedure allowed us to detect MPXV DNA in 56 wastewater samples collected from May 16 to August 4, 2022, with values ranging between 2.2 × 103 to 8.7 × 104 genome copies (gc)/L. This study shows that MPXV DNA can be reproducibly detected by qPCR in longitudinal samples collected from different Spanish wastewater treatment plants. According to data from the National Epidemiological Surveillance Network (RENAVE) in Spain a total of 6,119 cases have been confirmed as of August 19, 2022. However, and based on the wastewater data, the reported clinical cases seem to be underestimated and asymptomatic infections may be more frequent than expected.
This research was supported by the European Commission NextGenerationEU fund, through CSIC's Global Health Platform (PTI Salud Global), project CEX2021-001189-S MCIN/AEI / 10.13039/501100011033 and Fundación Séneca (Region of Murcia). Samples were obtained from the COVID-19 wastewater surveillance project (VATar COVID-19) funded by the Spanish Ministry for the Ecological Transition and the Demographic Challenge and the Spanish Ministry of Health.
With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2021-001189-S).
Databáze: OpenAIRE
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