Cartilage from the edge of a debrided articular defect is inferior to that from a standard donor site when used for autologous chondrocyte cultivation
| Autor: | E. Maličev, N. Kregar-Velikonja, M. Drobnič, K. Stražar, A. Barlič |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Cartilage Articular Pathology medicine.medical_specialty Knee Joint Biopsy Cell Culture Techniques Chondrocyte Lesion Arthroscopy Young Adult Chondrocytes Medicine Humans Orthopedics and Sports Medicine Autologous chondrocyte implantation Cell Shape Aggrecan biology medicine.diagnostic_test business.industry Cartilage medicine.anatomical_structure Debridement biology.protein Tissue and Organ Harvesting Versican Surgery medicine.symptom business |
| Zdroj: | The Journal of bone and joint surgery. British volume. 93(3) |
| ISSN: | 2044-5377 |
| Popis: | The aim of this study was to evaluate the cultivation potential of cartilage taken from the debrided edge of a chronic lesion of the articular surface. A total of 14 patients underwent arthroscopy of the knee for a chronic lesion on the femoral condyles or trochlea. In addition to the routine cartilage biopsy, a second biopsy of cartilage was taken from the edge of the lesion. The cells isolated from both sources underwent parallel cultivation as monolayer and three-dimensional (3D) alginate culture. The cell yield, viability, capacity for proliferation, morphology and the expressions of typical cartilage genes (collagen I, COL1; collagen II, COL2; aggrecan, AGR; and versican, VER) were assessed. The cartilage differentiation indices (COL2/COL1, AGR/VER) were calculated. The control biopsies revealed a higher mean cell yield (1346 cells/mg vs 341 cells/mg), but similar cell proliferation, viability and morphology compared with the cells from the edge of the lesion. The cartilage differentiation indices were superior in control cells: COL2/COL1 (threefold in biopsies (non-significant)); sixfold in monolayer cultures (p = 0.012), and 7.5-fold in hydrogels (non-significant), AGR/VER (sevenfold in biopsies (p = 0.04), threefold (p = 0.003) in primary cultures and 3.5-fold in hydrogels (non-significant)).Our results suggest that the cultivation of chondrocytes solely from the edges of the lesion cannot be recommended for use in autologous chondrocyte implantation. |
| Databáze: | OpenAIRE |
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