Multi-epitope based subunit vaccine construction against Banna virus targeting on two outer proteins (VP4 and VP9): A computational approach
| Autor: | Md. Mukthar Mia, Mahamudul Hasan, Md. Mahadi Hasan, Md. Nazmuj Sakib, Ankita Basak, Shakil Ahmed, Shrabonti Banik, Sumaya Shargin Khan, Mohammad Nahian Rahman |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Serotype Antigenicity Computational Biology Epitopes T-Lymphocyte Viral Vaccines Biology biology.organism_classification Microbiology Virology Virus Epitope Infectious Diseases Immune system Banna virus Vaccines Subunit Genetics Peptide vaccine biology.protein Epitopes B-Lymphocyte Capsid Proteins Antibody Molecular Biology Ecology Evolution Behavior and Systematics Coltivirus |
| Zdroj: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 95 |
| ISSN: | 1567-7257 |
| Popis: | Recently, RNA viruses have gained a mammoth concern for causing various outbreaks, and due to pandemics, they are acquiring additional attention throughout the world. An emerging RNA as well as vector-borne Banna Virus (BAV) is a human pathogen resulting in encephalitis, fever, headache, muscle aches, and severe coma. Besides human, pathogenic BAV was also detected from pigs, cattle, ticks, midges, and mosquitoes in Indonesia, China, and Vietnam. Due to high mutation tendency and dearth of a species barrier, this virus will consider as a significant threat in the near future throughout the planet, particularly in Africa. Despite of severe human case fatalities in several countries, there are no specific therapeutics, available vaccines, and other preventive measures against BAV. Thus, to find out the effective therapeutics and preventive strategies are crying exigency. In the present study, a unique multi-epitope-based peptide vaccine candidate is constructed using bioinformatics' tools that efficiently instigate immune cells for generating BAV antibodies. The potential vaccine candidates were developed using both T and B -cell epitopes. UniprotKB database was used to retrieve of two outer proteins (VP9 and VP4), and homologous sequences of BAV taxid: 7763, 649,604, 77,763, and 8453 were searched by NCBI BLAST. These serotypes are the most closely associated with the disease. Then combining the best-selected epitopes in various combinations with different adjuvants, three distinct vaccine candidates were formed. The validity tests were performed for the screened vaccine candidate regarding stability, allergenicity, and antigenicity parameters. Moreover, molecular dynamic simulations of the selected vaccine with TLR-8 immune receptor confirmed the stability of the binding pose and showed a significant response to immune cells. Thus, the results established that the designed chimeric peptide vaccine could enhance the immune response against BAV. |
| Databáze: | OpenAIRE |
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