Pre- and post-inspiratory neurons change their firing properties in female rats exposed to chronic intermittent hypoxia
| Autor: | Mateus R. Amorim, William H. Barnett, Benedito H. Machado, Ludmila Lima-Silveira, Yaroslav I. Molkov, Davi J. A. Moraes, George M. P. R. Souza |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Period (gene) SISTEMA RESPIRATÓRIO Neurotransmission Article 03 medical and health sciences 0302 clinical medicine Internal medicine Medicine Animals Respiratory system Rats Wistar Hypoxia Neurons Carotid Body business.industry General Neuroscience Excitatory Postsynaptic Potentials medicine.disease Rats Coupling (electronics) Obstructive sleep apnea 030104 developmental biology Endocrinology medicine.anatomical_structure nervous system Inhalation Excitatory postsynaptic potential Female Brainstem Neuron Nerve Net business 030217 neurology & neurosurgery |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Neuroscience |
| Popis: | Obstructive sleep apnea patients face episodes of chronic intermittent hypoxia (CIH), which has been suggested as a causative factor for increased sympathetic activity (SNA) and hypertension. Female rats exposed to CIH develop hypertension and exhibit changes in respiratory–sympathetic coupling, marked by an increase in the inspiratory modulation of SNA. We tested the hypothesis that enhanced inspiratory-modulation of SNA is dependent on carotid bodies (CBs) and are associated with changes in respiratory network activity. For this, in CIH-female rats we evaluated the effect of CBs ablation on respiratory–sympathetic coupling, recorded from respiratory neurons in the working heart–brainstem preparation and from NTS neurons in brainstem slices. CIH-female rats had an increase in peripheral chemoreflex response and in spontaneous excitatory neurotransmission in NTS. CBs ablation prevents the increase in inspiratory modulation of SNA in CIH-female rats. Pre-inspiratory/inspiratory (Pre-I/I) neurons of CIH-female rats have a reduced firing frequency. Post-inspiratory neurons are active for a longer period during expiration in CIH-female rats. Further, using the computational model of a brainstem respiratory–sympathetic network, we demonstrate that a reduction in Pre-I/I neuron firing frequency simulates the enhanced inspiratory SNA modulation in CIH-female rats. We conclude that changes in respiratory–sympathetic coupling in CIH-female rats is dependent on CBs and it is associated with changes in firing properties of specific respiratory neurons types. |
| Databáze: | OpenAIRE |
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