Myosin light chain phosphorylation during contraction of turtle heart
| Autor: | S.T. Sayers, K. Bárány |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Myosin light-chain kinase
Contraction (grammar) Phosphatase Biophysics Diastole Myosins Biology Contractility Biochemistry Structural Biology Myosin Genetics medicine Animals Cardiac muscle Phosphorylation Systole Molecular Biology Gel electrophoresis Myocardium Light chain phosphorylation Cell Biology Myosin light chain Myocardial Contraction Turtles Molecular Weight medicine.anatomical_structure |
| Zdroj: | FEBS Letters. 154:305-310 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(83)80172-0 |
| Popis: | The phosphorylation of myosin P-light chain was determined during the contraction cycle of turtle heart beating 5--8 times/min at 5 degrees C. The hearts were freeze-clamped either in systole or diastole, then homogenized and washed in strong acids in order to completely inhibit myosin light chain kinase and phosphatase and isolate the total P-light chain of the heart. The phospho and dephospho forms of P-light chain were separated by two-dimensional gel electrophoresis and were quantitated by densitometry. Alternatively, the hearts were perfused with 32P and the incorporation of [32P]phosphate into the P-light chain was determined. Both methods demonstrated that in hearts frozen in systole more P-light chain was phosphorylated than in hearts frozen in diastole. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text