3’UTR polymorphism of Thymidylate Synthase gene increased the risk of persistence of pre-neoplastic cervical lesions

Autor: Verlândia Mendes Nogueira, Ana Carolina da Silva Santos, Cláudia Martins Carneiro, Angélica Alves Lima, Nayara Nascimento Toledo Silva
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Adult
0301 basic medicine
Cancer Research
medicine.medical_specialty
HPV
Uterine Cervical Neoplasms
Genetic polymorphisms
5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
Polymorphism
Single Nucleotide
Gastroenterology
lcsh:RC254-282
Young Adult
03 medical and health sciences
0302 clinical medicine
Internal medicine
Genotype
Genetics
medicine
Humans
Methionine synthase
Allele
3' Untranslated Regions
Papillomaviridae
Aged
Cervical cancer
biology
TS3’UTR
business.industry
Papillomavirus Infections
Heterozygote advantage
(Methionine synthase) reductase
Middle Aged
medicine.disease
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
MTRR
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
Methylenetetrahydrofolate reductase
Pre-neoplastic cervical lesions
DNA
Viral
biology.protein
Thymidylate synthase
Female
business
Precancerous Conditions
Research Article
Zdroj: BMC Cancer, Vol 20, Iss 1, Pp 1-9 (2020)
BMC Cancer
ISSN: 1471-2407
DOI: 10.1186/s12885-020-06811-7
Popis: Background Cervical cancer is caused by high-risk Human Papillomavirus (hr-HPV) infection associated with cofactors that has been analyzed as predictors of the remission or persistence of cytological abnormalities remission or persistence. These cofactors can be either environmental, epigenetic, or genetic. Polymorphism in genes of enzymes that act on one-carbon metabolism alter their activity and also may be associated with cervical carcinogenesis because they affect DNA synthesis and repair, and gene expression. Therefore, this study aimed to analyze the risk of persistence of pre-neoplastic cervical lesions according to genetic polymorphisms involved in one-carbon metabolism. Methods Our sample consisted of 106 women, divided into two groups – Remission (n = 60), i.e., with the presence of pre-neoplastic lesions at first meeting (T1) and normal cytology after 6 months of follow-up (T2), and Persistence (n = 46), i.e., with the presence of pre-neoplastic lesions at T1 and T2. We obtained cervical samples for cytological analysis (T1 and T2), HPV detection (T1), and evaluation of polymorphism C667T of Methylenetetrahydrofolate Reductase (MTHFR C677T), A2756G of Methionine Synthase (MS A2756G), A66G of Methionine Synthase Reductase (MTRR A66G), double or triple 28 bp tandem repeat in 5′-untranslated enhanced region of Thymidylate Synthase (TSER), and 6 bp deletion at nucleotide 1494 in TS 3′-untranslated region (TS3’UTR). To analyze all genetic polymorphisms simultaneously, we calculated the Genetic Risk Score (GRS). Results We observed no differences between the Remission and Persistence groups regarding the GRS. Also, there were no differences in the genotypic and allelic distribution of MTHFR C677T and MS A2756G polymorphisms. However, the risk of persistence was higher among women with the heterozygote genotype - ins/del [OR (IC95%): 3.22 (1.19–8.69), p = 0.021], or the polymorphic genotype – del/del [OR (IC95%): 6.50 (1.71–24.70), p = 0.006] of TS3’UTR. Conclusions: The presence of the TS3’UTR polymorphism increased the risk of persistence of cervical abnormalities. This genetic variant could be a potential marker of cervical carcinogenesis and therefore assist the follow-up of women with persistent pre-neoplastic cervical lesions.
Databáze: OpenAIRE
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