Solid phase technology improves coupled gel shift/footprinting analysis
| Autor: | Anders Fjose, Peter B. Becker, Erlend Ragnhildstveit, Jean-Pierre Quivy |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Streptavidin
Resolution (mass spectrometry) Polyacrylamide DNA Footprinting Analytical chemistry Biotin DNA footprinting Biology Chemical Interference chemistry.chemical_compound Bacterial Proteins Phase (matter) Organometallic Compounds Genetics HSP70 Heat-Shock Proteins Promoter Regions Genetic DNA Footprinting DNA-Binding Proteins chemistry Biochemistry Electrophoresis Polyacrylamide Gel Sequence Analysis Copper Phenanthrolines Protein Binding Research Article |
| Zdroj: | Nucleic Acids Research. 25:453-454 |
| ISSN: | 1362-4962 |
| DOI: | 10.1093/nar/25.2.453 |
| Popis: | For the analysis of protein-DNA interactions by coupled gel-shift/footprinting, DNA fragments need to be extracted from polyacrylamide gels and subsequently separated on high resolution gels. Due to impurities in the extracted DNA, single nucleotide resolution is frequently not achieved. We now describe an improved experimental strategy that employs transient coupling of DNA fragments to a solid support in order to extract DNA of high purity quantitatively, rapidly and reliably. As an example, we describe the application of our protocol to the 'in-gel footprinting' by copper phenanthroline. The method should also find application to the chemical interference assays. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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