Insect mucin-type glycoprotein: Immunodetection of the O-glycosylated epitope in Drosophila melanogaster cells and tissues
| Autor: | Rozovsky Ya M, Pochechueva Tv, N.A. Baikova, Vladimir A. Gvozdev, Elena A. Mikhaleva, Kramerov Aa, Arsenjeva El |
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| Rok vydání: | 1997 |
| Předmět: |
Glycosylation
animal structures medicine.drug_class Biology Monoclonal antibody Biochemistry Epitope Cell Line Epitopes chemistry.chemical_compound medicine Animals Molecular Biology chemistry.chemical_classification Mucin Mucins Antibodies Monoclonal biology.organism_classification Molecular biology Drosophila melanogaster chemistry Cell culture Insect Science biology.protein Antibody Glycoprotein |
| Zdroj: | Insect Biochemistry and Molecular Biology. 27:513-521 |
| ISSN: | 0965-1748 |
| DOI: | 10.1016/s0965-1748(97)00026-x |
| Popis: | A mucin-type glycoprotein (GP) from cultured embryonic cells of Drosophila melanogaster was isolated and used to raise monoclonal antibodies (MAbs). Epitope(s) recognized by MAbs were sensitive to the treatment by O-glycanase, which specifically cleaves off O-linked mucin-type Gal(beta 1,3)GalNAc disaccharide, representing the major part of the carbohydrate moiety of Drosophila GP. Using high-affinity MAbs against carbohydrate epitopes of the Drosophila mucin GP we demonstrated its accumulation in culture medium, as well as in cultured cells, which proved to be regulated by 20-hydroxyecdysone. Mucin GPs carrying Gal(beta 1,3)GalNAc disaccharide recognized by the MAbs were immunochemically localized in several Drosophila tissues of ectodermal, mesodermal and germ line origin, including epidermal and follicle cells capable of their secretion. |
| Databáze: | OpenAIRE |
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