Gene alteration and precursor and mature microRNA transcription changes contribute to the miRNA signature of primary effusion lymphoma
| Autor: | Wolfgang Vahrson, Andrea J. O'Hara, Dirk P. Dittmer |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Lymphoma
Transcription Genetic Immunology Biology Biochemistry RNA interference Cell Line Tumor hemic and lymphatic diseases microRNA Transcriptional regulation medicine Humans Gene silencing RNA Neoplasm Copy-number variation Gene Neoplasia Cell Biology Hematology medicine.disease Immunohistochemistry Molecular biology Neoplasm Proteins Cell biology MicroRNAs Real-time polymerase chain reaction Mutation Primary effusion lymphoma |
| Zdroj: | Blood. 111:2347-2353 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood-2007-08-104463 |
| Popis: | MicroRNAs are regulated by gene alteration, transcription, and processing. Thus far, few studies have simultaneously assessed all 3 levels of regulation. Using real-time quantitative polymerase chain reaction (QPCR)–based arrays, we determined changes in gene copy number, pre-miRNA, and mature miRNA levels for the largest set of primary effusion lymphomas (PELs) to date. We detected PEL-specific miRNA gene amplifications, and concordant changes in pre-miRNA and mature miRNA. We identified 68 PEL-specific miRNAs. This defines the miRNA signature of PEL and shows that transcriptional regulation of pre-miRNA as well as mature miRNA levels contribute nonredundant information that can be used for the classification of human tumors. |
| Databáze: | OpenAIRE |
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