Protection against Vibrio alginolyticus in crimson snapper Lutjanus erythropterus immunized with a DNA vaccine containing the ompW gene
| Autor: | Yu Ding, Jufen Tang, Yu-Cong Huang, Zaohe Wu, Bei Wang, Jichang Jian, Yishan Lu, Shuanghu Cai |
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| Rok vydání: | 2013 |
| Předmět: |
Lutjanus erythropterus
Virulence Aquatic Science law.invention DNA vaccination Microbiology Fish Diseases Plasmid Bacterial Proteins law Vaccines DNA Animals Gene Ecology Evolution Behavior and Systematics Vibrio alginolyticus biology Gene Expression Regulation Bacterial biology.organism_classification Virology Perciformes Vaccination Vibrio Infections Bacterial Vaccines Recombinant DNA Bacterial Outer Membrane Proteins |
| Zdroj: | Diseases of aquatic organisms. 106(1) |
| ISSN: | 0177-5103 |
| Popis: | The outer membrane proteins of Vibrio alginolyticus play an important role in the virulence of the bacterium and are potential candidates for vaccine development. In the present study, the ompW gene was cloned, expressed and purified. A DNA vaccine was constructed by inserting the ompW gene into a pcDNA plasmid. Crimson snapper Lutjanus erythropterus (Bloch) were injected intramuscularly with the recombinant plasmid pcDNA-ompW. The expression of the DNA vaccine was detected in gill, head kidney, heart, liver, spleen and injection site muscle of crimson snapper by RT-PCR 7 and 28 d post-vaccination. The ELISA results demonstrated that the DNA vaccine produced an observable antibody response in all sera of the vaccinated fish. In addition, crimson snapper immunized with the DNA vaccine showed a relative percentage survival (RPS) of 92.53%, indicating effective protection against V. alginolyticus infection. |
| Databáze: | OpenAIRE |
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