Determination of 20, 25-diazacholesterol in avian matrices by high performance liquid chromatography/tandem mass spectrometry
| Autor: | Laura E. Hulslander, Benjamin G. Abbo, David A. Goldade |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Cholesterol synthesis Clinical Biochemistry Relative standard deviation Tandem mass spectrometry Mass spectrometry 010603 evolutionary biology 01 natural sciences Biochemistry High-performance liquid chromatography Analytical Chemistry Birds Contraceptive Agents Limit of Detection Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Animals Tissue Distribution Solid phase extraction Chromatography High Pressure Liquid Detection limit Chromatography Chemistry Solid Phase Extraction Reproducibility of Results Cell Biology General Medicine 010601 ecology Liver Linear Models Pest Control Azacosterol |
| Zdroj: | Journal of Chromatography B. :129-133 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2017.09.028 |
| Popis: | Wildlife contraceptives are an emerging tool for minimizing human-wildlife conflicts. One promising avian contraceptive compound, 20,25-diazacholesterol (DAC), reduces fertility by inhibiting cholesterol synthesis. A reliable analytical method for DAC was required in support of its registration for use as a reproductive control agent in pest bird species. A liquid chromatographic method employing tandem mass spectrometry (LC-MS/MS) was developed for the analysis of tissue extracts following solid phase extraction clean-up. Tissues analyzed were whole body samples from crows, monk parakeets, and quails and liver samples from crows and quails. Excellent sensitivity and selectivity was afforded by tandem mass spectrometry. The method accuracy of DAC from various tissue samples fortified at parts-per-million (ppm) and parts-per-billion (ppb) concentrations was high (>90%) with excellent precision ( |
| Databáze: | OpenAIRE |
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