Screening yeast artificial chromosome libraries with robot-aided automation
| Autor: | David D. Sloan, F. Burough, Volker Nowotny, Mary M. Blanchard |
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| Rok vydání: | 1993 |
| Předmět: |
Yeast artificial chromosome
Chromatography X Chromosome Pcr assay Pipette DNA Robotics Biology Applied Microbiology and Biotechnology Molecular biology DNA extraction Polymerase Chain Reaction Yeast law.invention Globins chemistry.chemical_compound chemistry law Genetics Humans Chromosomes Artificial Yeast Polymerase chain reaction Software Sequence Tagged Sites |
| Zdroj: | Genetic analysis, techniques and applications. 10(6) |
| ISSN: | 1050-3862 |
| Popis: | Screening of collections of yeast artificial chromosomes utilizing the polymerase chain reaction (PCR) requires large numbers of reactions in parallel. Four steps were implemented to reduce the labor involved: (a) The number of initial samples for DNA extractions was decreased by compressing libraries up to 12-fold. (b) DNA extraction from yeast clones was robot assisted. (c) A Biomek 1000 station was adapted to pipette samples for PCR assays. (d) Sample preparation was integrated with a temperature cycler constructed to carry out up to 576 reactions in six 8 × 12-well trays. The implementation of these steps increases the number of reactions per person per day by an order of magnitude. In tests with X-chromosome-specific probes, the robot-aided screening recovered all of the clones detected by slower manual methods. |
| Databáze: | OpenAIRE |
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