The cell envelope structure and properties of Mycobacterium smegmatis mc2155 : is there a clue for the unique transformability of the strain ?
| Autor: | Françoise Laval, Ahmed Abouwarda, Premkumar Dinadayala, Alexandra Galamba, Gilles Etienne, Didier Zerbib, Mamadou Daffé, Christelle Villeneuve |
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| Přispěvatelé: | Institut de pharmacologie et de biologie structurale (IPBS), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Lipopolysaccharides
Mutant Mycobacterium smegmatis Chenodeoxycholic Acid Microbiology Permeability Cell wall 03 medical and health sciences Glycolipid Microscopy Electron Transmission Cell Wall Chenodeoxycholate Phospholipids 030304 developmental biology 0303 health sciences Strain (chemistry) biology 030306 microbiology Cell Membrane Glycopeptides Biological Transport biology.organism_classification [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Biochemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Biophysics Ultrastructure Chromatography Thin Layer Cell envelope Glycolipids |
| Zdroj: | Microbiology (Reading, England) Microbiology (Reading, England), 2005, 151, pp.2075-2086 |
| Popis: | Mycobacterium smegmatisis often used as a surrogate host for pathogenic mycobacteria, especially since the isolation of the transformable smooth morphotype strain mc2155 from the isogenic rough wild-type strain ATCC 607. Biochemical analysis of the cell envelope components revealed a lack of polar glycolipids, namely the lipooligosaccharides and the polar subfamilies of glycopeptidolipids, in the mc2155 strain. In addition, the latter strain differs from its parent by the distribution of various species of glycolipids and phospholipids between the outermost and deeper layers of the cell envelope. The presence of filamentous and rope-like structures at the cell surface of mc2155 cells grown in complex media further supported an ultrastructural change in the cell envelope of the mutant. Importantly, a significantly more rapid uptake of the hydrophobic chenodeoxycholate was observed for the mutant compared to wild-type cells. Taken together, these data indicate that the nature of the surface-exposed and envelope constituents is crucial for the surface properties, cell wall permeability and bacterial phenotype, and suggest that the transformable character of the mc2155 strain may be in part explained by these profound modifications of its cell envelope. |
| Databáze: | OpenAIRE |
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