| Autor: |
Marios A. Cariolou, Constantin N. Flytzanis |
| Rok vydání: |
1994 |
| Předmět: |
|
| Zdroj: |
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry. 108:367-373 |
| ISSN: |
0305-0491 |
| DOI: |
10.1016/0305-0491(94)90088-4 |
| Popis: |
High-resolution mini-two-dimensional polyacrylamide gel electrophoresis (mini-2D-PAGE) was used to analyze silver-stained, soluble proteins from the cuticle-epidermis of Penaeus vannamei during molting. The 2D-PAGE patterns of epidermis polypeptides from metecdysis and anecdysis/proecdysis molt stages demonstrated similarities as well as several quantitative and qualitative differences. Quantitative modulation in polypeptide expression was noted in at least seven prevalent polypeptides during molting. A 50 kDa protein is specifically expressed in anecdysis/proecdysis tissue samples. Quantitative and qualitative differences were also noted in proteins migrating mainly in the molecular mass ranges of 26–32 kDa. An overall increase in polypeptide expression was noted in this molecular mass range at metecdysis as compared to anecdysis/proecdysis epidermis tissues. These results indicate modulation of cuticle-epidermis proteins in Penaeus vannamei shrimps during molting. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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