Fluorescent Properties of Carboxyfluorescein Bifluorophores
| Autor: | Fan Fan, Sergei A. Tikhomirov, Pham Van Duong, Aliaksandr S Kruhlik, Vadim V. Shmanai, V. A. Povedailo, Dmitrii L Yakovlev, Ivan L. Lysenko, O. L. Sharko, Pham Thi Hong Minh, Dmitry A. Tsybulsky, Yury V Martynenko-Makaev |
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| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Sociology and Political Science 010405 organic chemistry Chemistry Biomolecule Clinical Biochemistry Rigid structure 010402 general chemistry Branching (polymer chemistry) 01 natural sciences Biochemistry Fluorescence 0104 chemical sciences Clinical Psychology chemistry.chemical_compound Click chemistry Biophysics Fluorescein Law Native structure Linker Spectroscopy Social Sciences (miscellaneous) |
| Zdroj: | Journal of fluorescence. 30(3) |
| ISSN: | 1573-4994 |
| Popis: | Bright fluorescent probes with enhanced intensities in the fluorescein channel are of great value for plenty of biological applications. To design effective probes one should introduce as many as possible fluorophores to the biomolecule while leaving its native structure as intact as possible. To reach this compromise, we designed and synthesized fluorescein bifluorophores on the 3,5-diaminobenzoic acid scaffold, which allows for insertion of two fluorophores at one modification site of a biomolecule. Rigid structure of the branching linker group allows to minimize self-quenching the fluorophores. However, despite the structure similarities of fluorescein isomers (5-FAM and 6-FAM), different photophysical behavior was observed for the corresponding bifluorophores. Here we made efforts to get insight into these effects with the focus on the media viscosity impact. |
| Databáze: | OpenAIRE |
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