Quantitative proteomic analysis of Xanthoceras sorbifolium Bunge seedlings in response to drought and heat stress
| Autor: | Wei Du, Xin Jiang, Siyang Zhao, Li Jingbin, Jian Ding, Cheng-Jiang Ruan, He Li |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hot Temperature Proteome Photosystem II Physiology Plant Science Oxidative phosphorylation Photosystem I 01 natural sciences Superoxide dismutase 03 medical and health sciences Sapindaceae Stress Physiological Heat shock protein Genetics chemistry.chemical_classification Reactive oxygen species biology NADH dehydrogenase food and beverages Droughts 030104 developmental biology chemistry Biochemistry Seedlings biology.protein Heat-Shock Response 010606 plant biology & botany Isobaric tag for relative and absolute quantitation |
| Zdroj: | Plant Physiology and Biochemistry. 160:8-17 |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/j.plaphy.2021.01.002 |
| Popis: | Yellowhorn (Xanthoceras sorbifolium Bunge) is a woody oil species that is widely distributed in northwestern China. To investigate the molecular mechanisms underlying the drought and heat tolerance response of yellowhorn seedlings, changes in protein abundance were analyzed via comparative proteomics. Drought and heat treatment of seedlings was applied in growth chamber, and the leaves were harvested after 7 days of treatment. The total protein was extracted, and comparative proteomic analysis was performed via isobaric tag for relative and absolute quantitation (iTRAQ). The abundance of most of the proteins associated with oxidative phosphorylation, NADH dehydrogenase and superoxide dismutase (SOD) was reduced. The differential proteins associated with photosynthesis enzymes indicated that stress had different effects on photosystem I (PSI) and photosystem II (PSII). After comprehensively analyzing the results, we speculated that drought and heat stress could hinder the synthesis of riboflavin, reducing NADH dehydrogenase content, which might further have an impact on energy utilization. Yellowhorn seedlings relied on Fe-Mn SOD enzymes rather than Cu/Zn SOD enzymes to remove reactive oxygen species (ROS). In addition, heat-shock proteins (HSPs) had significant increase and played a key role in stress response, which could be divided into two categories according to their transcription and translation efficiency. Over all, the results can provide a basis for understanding the molecular mechanism underlying resistance to drought and heat stress in yellowhorn and for subsequent research of posttranslational modification-related omics of key proteins. |
| Databáze: | OpenAIRE |
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