Liquid Chromatographic Determination of NSC 737664 (ABT-888: An Inhibitor of Poly(ADP-ribose) Polymerase (PARP)) in Plasma and Urine in a Phase 0 Clinical Trial
| Autor: | Kimberly Hill, Eva Majerova, Lawrence R. Phillips |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Electrospray
Chromatography Formic acid Electrospray ionization Clinical Biochemistry Pharmaceutical Science Urine Reversed-phase chromatography Mass spectrometry Biochemistry High-performance liquid chromatography Article Analytical Chemistry chemistry.chemical_compound chemistry Quantitative analysis (chemistry) |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 32:261-272 |
| ISSN: | 1520-572X 1082-6076 |
| DOI: | 10.1080/10826070802603351 |
| Popis: | A gradient reversed-phase high performance liquid chromatographic method was developed for determining NSC 737664 (2-[(2R)-2-methylpyrrolidin-2-yl]-1H-benzimidazole-4-carboxamide; ABT-888) in human plasma and urine. Chromatographic separation used a mobile phase composed of 0.1% formic acid in water and 0.1% formic acid in acetonitrile, and a C18 column (150 mm × 4.6 mm, 5µ). Quantitation was performed using UV detection at 300 nm. Chromatographic peak identity was confirmed using positive-ion electrospray ionization mass spectrometry. The method was shown to be specific, accurate and reproducible, and thereby appropriate for monitoring plasma and urine levels of the agent in support of a phase 0 clinical study. |
| Databáze: | OpenAIRE |
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