Rhythmic expression of the cycle gene in a hematophagous insect vector
| Autor: | Antonio C. A. Meireles-Filho, Charalambos P. Kyriacou, Nataly A. Souza, Alexandre A. Peixoto, Paulo Roberto de Amoretty |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
DNA
Complementary lcsh:QH426-470 Circadian clock ved/biology.organism_classification_rank.species Molecular Sequence Data Genes Insect Transcription (biology) Cricetinae Anopheles Animals Circadian rhythm Amino Acid Sequence RNA Messenger Cloning Molecular Phosphorylation lcsh:QH573-671 Model organism Molecular Biology Gene Phylogeny Genetics Binding Sites biology Sequence Homology Amino Acid ved/biology Reverse Transcriptase Polymerase Chain Reaction lcsh:Cytology Gene Expression Profiling Sequence Analysis DNA biology.organism_classification Bombyx Cell biology Circadian Rhythm Insect Vectors Gene expression profiling lcsh:Genetics Trans-Activators Insect Proteins Female Drosophila melanogaster Psychodidae Research Article |
| Zdroj: | BMC Molecular Biology, Vol 7, Iss 1, p 38 (2006) BMC Molecular Biology |
| ISSN: | 1471-2199 |
| Popis: | Background A large number of organisms have internal circadian clocks that enable them to adapt to the cyclic changes of the external environment. In the model organism Drosophila melanogaster, feedback loops of transcription and translation are believed to be crucial for the maintenance of the central pacemaker. In this mechanism the cycle (or bmal1) gene, which is constitutively expressed, plays a critical role activating the expression of genes that will later inhibit their own activity, thereby closing the loop. Unlike Drosophila, the molecular clock of insect vectors is poorly understood, despite the importance of circadian behavior in the dynamic of disease transmission. Results Here we describe the sequence, genomic organization and circadian expression of cycle in the crepuscular/nocturnal hematophagous sandfly Lutzomyia longipalpis, the main vector of visceral leishmaniasis in the Americas. Deduced amino acid sequence revealed that sandfly cycle has a C-terminal transactivation domain highly conserved among eukaryotes but absent in D. melanogaster. Moreover, an alternative form of the transcript was also identified. Interestingly, while cycle expression in Drosophila and other Diptera is constitutive, in sandflies it is rhythmic in males and female heads but constitutive in the female body. Blood-feeding, which causes down-regulation of period and timeless in this species, does not affect cycle expression. Conclusion Sequence and expression analysis of cycle in L. longipalpis show interesting differences compared to Drosophila suggesting that hematophagous vector species might present interesting new models to study the molecular control of insect circadian clocks. |
| Databáze: | OpenAIRE |
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