Investigation of lymphoid lesions of the head and neck using combined fine needle aspiration cytology and flow cytometry: Accuracy and pitfalls

Autor:	Mary Anne Kalokerinos, Peter Hobson, Paul Shield, Heinrich Crous, Sasenka Knezevic, David Papadimos, Amanda Gillam
Rok vydání:	2019
Předmět:	Adult Male Pathology medicine.medical_specialty Histology Adolescent T-Lymphocytes Biopsy Fine-Needle Follicular lymphoma 030209 endocrinology & metabolism Lymphoma T-Cell Pathology and Forensic Medicine 03 medical and health sciences Young Adult 0302 clinical medicine immune system diseases hemic and lymphatic diseases Cytology medicine Humans Child Lymphoma Follicular Aged Aged 80 and over B-Lymphocytes medicine.diagnostic_test business.industry Large cell Lymphoma Non-Hodgkin Not Otherwise Specified Infant General Medicine Lymphoma B-Cell Marginal Zone Hyperplasia Middle Aged medicine.disease Marginal zone Flow Cytometry Hodgkin Disease Lymphoma Fine-needle aspiration 030220 oncology & carcinogenesis Child Preschool Female business
Zdroj:	Cytopathology : official journal of the British Society for Clinical Cytology. 30(4)
ISSN:	1365-2303
Popis:	We reviewed the diagnostic utility of combined fine needle aspiration cytology (FNAC) and flow cytometry (FC) in the diagnosis of lymphoid lesions of the head and neck.In total, 1402 patients with combined FNAC-FC reports were correlated with follow-up information. Rapid on-site evaluation (ROSE) of cytological specimens was performed in 52% of cases.In total, 211 lymphoid malignancies were identified, including 198 non-Hodgkin lymphoma (NHL) and 13 Hodgkin lymphoma (HL). Accuracy measures for NHL were: sensitivity 95.5%; specificity 99.9%; PPV 99.5%; NPV 99.2%; accuracy 99.3%. Only seven of 13 cases of HL were detected by FNAC-FC. False negative cases included HL (six cases), diffuse large B-cell lymphoma (four), T-cell lymphoma (two), follicular lymphoma (one), marginal zone cell lymphoma (one) and B-cell NHL, not otherwise specified (one). Two false positive results were identified: one immunoblastic hyperplasia reported as suspicious for HL and one case reported as suggestive of NHL that was found to be reactive hyperplasia. Cases collected with ROSE had a significantly lower rate (P 0.0001) of insufficient cells for FC analysis (7.0%) than cases where ROSE was not performed (16.4%). Sensitivity (P 0.0001) and NPV (P = 0.0023) were significantly higher for ROSE-collected specimens. None of the false-negative NHL cases had ROSE performed.FNAC-FC is a highly sensitive and specific test for NHL. Diagnostic errors mostly involved HL, large cell lymphomas and T-cell lymphomas. ROSE results in a significantly higher adequacy rate for FC and higher sensitivity for NHL.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f0045881ccb8ba93d8a45af299e88ae https://pubmed.ncbi.nlm.nih.gov/30985042 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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