Critical points in charged membranes containing cholesterol
| Autor: | Harden M. McConnell, Arun Radhakrishnan |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Membrane lipids
Static Electricity Thermodynamics G(M1) Ganglioside In Vitro Techniques Miscibility Critical point (mathematics) Membrane Lipids Freezing Monolayer Pressure Animals Organic chemistry Phase diagram Sheep Multidisciplinary Chemistry Osmolar Concentration Unithiol Hydrogen-Ion Concentration Cholestanol Cholesterol Membrane Microscopy Fluorescence Ionic strength Physical Sciences lipids (amino acids peptides and proteins) Ternary operation |
| Zdroj: | Proceedings of the National Academy of Sciences. 99:13391-13396 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.212522699 |
| Popis: | Epifluorescence microscopy is used to determine phase diagrams for lipid monolayers containing binary mixtures of cholesterol or dihydrocholesterol and dimyristoylphosphatidylserine, as well as ternary mixtures that also contain ganglioside G M1 . The phase diagrams are unusual in that they show multiple critical points: two upper miscibility critical points and one lower miscibility critical point. These critical points all are associated with the formation of condensed complexes between these lipids and cholesterol (or dihydrocholesterol). The miscibility critical pressures depend on subphase pH and ionic strength. Changes in critical pressures due to changes in subphase composition are closely related to changes in membrane electrostatic pressure and surface ionization. |
| Databáze: | OpenAIRE |
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