Transcriptional and post-transcriptional regulation of a NAC1 transcription factor in Medicago truncatula roots
| Autor: | Sofie Goormachtig, Stefanie De Bodt, Annick De Keyser, Katrien D'haeseleer, Carole Laffont, Griet Den Herder, Florian Frugier, Martin Crespi, Virginie Mortier, Christine Lelandais-Brière, Julie Plet, Marcelle Holsters |
|---|---|
| Přispěvatelé: | Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Center for Plant Systems Biology (PSB Center), Vlaams Instituut voor Biotechnologie [Ghent, Belgique] (VIB) |
| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
Physiology MESH: Plant Shoots Arabidopsis MESH: Plant Roots MESH: Amino Acid Sequence Plant Science MESH: RNA Plant MESH: Base Sequence 01 natural sciences Plant Root Nodulation Plant Roots MESH: Protein Structure Tertiary Gene Expression Regulation Plant Arabidopsis thaliana MESH: Arabidopsis MESH: Phylogeny MESH: Tobacco MESH: Medicago truncatula Lateral root formation Phylogeny Plant Proteins Regulation of gene expression 0303 health sciences MESH: Plant Proteins biology food and beverages MESH: Transcription Factors Plants Genetically Modified MESH: Saccharomyces cerevisiae Medicago truncatula Cell biology MESH: Plant Leaves RNA Plant MESH: Plant Root Nodulation Plant Shoots MESH: Indoleacetic Acids MESH: Mutation MESH: Trans-Activators Recombinant Fusion Proteins Molecular Sequence Data MESH: Arabidopsis Proteins Flowers Saccharomyces cerevisiae Rhizobia 03 medical and health sciences Botany Tobacco MESH: Recombinant Fusion Proteins [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Amino Acid Sequence MESH: Gene Expression Regulation Plant Transcription factor 030304 developmental biology MESH: Molecular Sequence Data Base Sequence Indoleacetic Acids Arabidopsis Proteins fungi Lateral root MESH: Flowers biology.organism_classification Protein Structure Tertiary Plant Leaves MicroRNAs MESH: Plants Genetically Modified Mutation Trans-Activators MESH: Sinorhizobium meliloti MESH: MicroRNAs 010606 plant biology & botany Sinorhizobium meliloti Transcription Factors |
| Zdroj: | New Phytologist New Phytologist, Wiley, 2011, 191 (3), pp.647-61. ⟨10.1111/j.1469-8137.2011.03719.x⟩ |
| ISSN: | 1469-8137 0028-646X |
| DOI: | 10.1111/j.1469-8137.2011.03719.x⟩ |
| Popis: | International audience; Legume roots develop two types of lateral organs, lateral roots and nodules. Nodules develop as a result of a symbiotic interaction with rhizobia and provide a niche for the bacteria to fix atmospheric nitrogen for the plant. * The Arabidopsis NAC1 transcription factor is involved in lateral root formation, and is regulated post-transcriptionally by miRNA164 and by SINAT5-dependent ubiquitination. We analyzed in Medicago truncatula the role of the closest NAC1 homolog in lateral root formation and in nodulation. * MtNAC1 shows a different expression pattern in response to auxin than its Arabidopsis homolog and no changes in lateral root number or nodulation were observed in plants affected in MtNAC1 expression. In addition, no interaction was found with SINA E3 ligases, suggesting that post-translational regulation of MtNAC1 does not occur in M. truncatula. Similar to what was found in Arabidopsis, a conserved miR164 target site was retrieved in MtNAC1, which reduced protein accumulation of a GFP-miR164 sensor. Furthermore, miR164 and MtNAC1 show an overlapping expression pattern in symbiotic nodules, and overexpression of this miRNA led to a reduction in nodule number. * This work suggests that regulatory pathways controlling a conserved transcription factor are complex and divergent between M. truncatula and Arabidopsis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text