Insights into regulatory characteristics of the promoters of Sericin 1 and Sericin 3 in transgenic silkworms
| Autor: | Tingting Tan, Rongpeng Liu, Hanfu Xu, Yao Ou, Wenhui Zeng, Qin Luo, Dawei Qu, Jingwen Ma |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Transgene Green Fluorescent Proteins Biophysics Biology Biochemistry Sericin Green fluorescent protein Animals Genetically Modified 03 medical and health sciences 0302 clinical medicine Animals Sericins Promoter Regions Genetic Molecular Biology Gene Silk gland fungi Pupa Gene Expression Regulation Developmental Promoter Cell Biology Bombyx Cell biology 030104 developmental biology SILK Larva 030220 oncology & carcinogenesis |
| Zdroj: | Biochemical and Biophysical Research Communications. 522:492-498 |
| ISSN: | 0006-291X |
| Popis: | Sericin, produced in the middle silk gland (MSG) of silkworms, is a group of glue proteins that coat and cement silk fibers. Several genes are known to encode sericin, but their spatiotemporal regulation has yet to be fully elucidated. Here, we report in detail the expression profiles of the promoters of two major sericin-coding genes, Sericin 1 (Ser1)and Sericin 3 (Ser3), by analyzing Gal4/UAS transgenic silkworms. We found that UAS-linked EGFP fluorescence in transgenic silkworms driven by Ser1-Gal4was detected in only the R3, R4 and R5 regions of MSG starting inday-3 fifth-instar larvae and was continuously expressed until silk gland degradation. In transgenic silkworms driven by Ser3-Gal4, EGFP fluorescence was detected at a low level in the R2 region of MSG since the last day of fifth-instar larvae, and the expression increased during the wandering stages and was continuously detected until silk gland degradation. The molecular detection of EGFP expression in each of the Gal4/UAS transgenic silkworms was consistent with fluorescence observations. These findings reveal clear differences in the regulatory characteristics of the promoters of Ser1and Ser3 and provide new insights into the regulatory mechanism of the expression of sericin-coding genes. |
| Databáze: | OpenAIRE |
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