Fluorescence hysteroscopy using 5-aminolevulinic: A descriptive study
| Autor: | Rene Hornung, Rosmarie Caduff, Pius Wyss, Andrea Degen, Mathias K. Fehr, Urs Haller |
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| Přispěvatelé: | University of Zurich, Wyss, Pius |
| Rok vydání: | 2003 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Administration Topical medicine.medical_treatment H&E stain 340 Law Pilot Projects Hysteroscopy Dermatology Endometrium Fluorescence 2708 Dermatology chemistry.chemical_compound medicine Carcinoma Atypia Humans Aged Aged 80 and over Photosensitizing Agents medicine.diagnostic_test Eosin business.industry Aminolevulinic Acid Middle Aged medicine.disease 10174 Clinic for Gynecology Curettage Endometrial Neoplasms 2746 Surgery medicine.anatomical_structure chemistry Endometrial Hyperplasia Feasibility Studies Female Surgery Uterine cavity Nuclear medicine business |
| Zdroj: | Lasers in Surgery and Medicine. 33:209-212 |
| ISSN: | 1096-9101 0196-8092 |
| Popis: | Background and Objectives To assess the feasibility of fluorescence hysteroscopy following topical ALA application and to descript the optical appearance of normal and neoplastic endometrium. Study Design/Materials and Methods Fifty-four patients scheduled for routine hysteroscopy and D&C were assigned to the study group using topically applied ALA. Two milliliters of a 2% 5-ALA-solution at pH = 4.0 (ASAT AG/Zug, Switzerland) was administrated into the uterine cavity 4 hours before fluorescence hysteroscopy, using a STORZ-D-Light (Tuttlingen, Germany) system. Hysteroscopic findings were compared to the histological diagnosis of hematoxylin & eosin (H&E) stained sections. In the study group, 60 histological specimens were taken (47 materials of curettage and 13 targeted biopsies). The percentage of fluorescence positive patients was calculated. Results The rate of fluorescent endometrial samples was low in atrophic endometrium 1/16 (6.2%). Endometrial carcinoma were diagnosed in two patients. Both cases exhibited selective and intense PpIX-mediated red fluorescence. Fluorescence hysteroscopy could not differentiate between hyperplastic areas with or without atypia. Minimal uterine cramps or discomfort following ALA instillation were complained by 30 (56%) patients, no pain was indicated by 24 patients (44%). Conclusions Fluorescence hysteroscopy following intrauterine application of ALA is feasible. Cancerous, hyperplastic, and secretory endometrial tissue showed high red fluorescence. Lasers Surg. Med. 33:209–212, 2003. © 2003 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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