Nascent RNA antagonizes the interaction of a set of regulatory proteins with chromatin
Autor: | Igor Ruiz de los Mozos, Garima Khandelwal, Till Bartke, Amandeep Bhamra, Victoria Begley, Rachel Wellman, Javier Herrero, Manuel Tavares, Silvia Surinova, Benjamin M. Foster, Andrey Tvardovskiy, Manuel Beltran, Saulius Lukauskas, Richard G. Jenner, Ambrosius P. Snijders, Lenka Skalska, Peter Faull |
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Rok vydání: | 2020 |
Předmět: |
Male
Proteomics Transcription Genetic RNase P RNA polymerase II Article 03 medical and health sciences Mice 0302 clinical medicine 7SK RNA Nucleosome Animals Humans Positive Transcriptional Elongation Factor B Molecular Biology Transcription factor Embryonic Stem Cells 030304 developmental biology 0303 health sciences biology RNA Cell Biology Chromatin Cell biology Nucleosomes DNA-Binding Proteins Rna Polymerase Ii Rnase Histone Modification Nascent Rna Nucleosome Remodeller Pre-mrna Transcription Factor Transcriptional Elongation Histone HEK293 Cells Gene Expression Regulation biology.protein RNA Polymerase II Transcriptional Elongation Factors 030217 neurology & neurosurgery Protein Binding Transcription Factors |
Zdroj: | Mol Cell Mol. Cell 81, 2944-2959.e10 (2021) |
ISSN: | 1097-4164 |
Popis: | A number of regulatory factors are recruited to chromatin by specialized RNAs. Whether RNA has a more general role in regulating the interaction of proteins with chromatin has not been determined. We used proteomics methods to measure the global impact of nascent RNA on chromatin in embryonic stem cells. Surprisingly, we found that nascent RNA primarily antagonized the interaction of chromatin modifiers and transcriptional regulators with chromatin. Transcriptional inhibition and RNA degradation induced recruitment of a set of transcriptional regulators, chromatin modifiers, nucleosome remodelers, and regulators of higher-order structure. RNA directly bound to factors, including BAF, NuRD, EHMT1, and INO80 and inhibited their interaction with nucleosomes. The transcriptional elongation factor P-TEFb directly bound pre-mRNA, and its recruitment to chromatin upon Pol II inhibition was regulated by the 7SK ribonucleoprotein complex. We postulate that by antagonizing the interaction of regulatory proteins with chromatin, nascent RNA links transcriptional output with chromatin composition. |
Databáze: | OpenAIRE |
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