Comprehensive Analysis of Soybean Mosaic Virus P3 Protein Interactors and Hypersensitive Response-Like Lesion-Inducing Protein Function
| Autor: | Xin Chen, Haijian Zhi, Hexiang Luan, Wenlin Liao, Haopeng Niu, Xiaoyan Cui |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hypersensitive response Genetic Vectors Potyvirus Soybean mosaic virus Biology 01 natural sciences Catalysis Virus Article Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences Bimolecular fluorescence complementation Viral Proteins soybean mosaic virus Gene expression Gene Order Protein Interaction Mapping cDNA library screening Amino Acid Sequence Protein Interaction Maps Physical and Theoretical Chemistry soybean Molecular Biology lcsh:QH301-705.5 Spectroscopy Phylogeny Plant Diseases cDNA library Gene Expression Profiling Organic Chemistry High-Throughput Nucleotide Sequencing P3 General Medicine Periplasmic space Subcellular localization biology.organism_classification Computer Science Applications Cell biology hypersensitive response like lesion inducing 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Host-Pathogen Interactions Carrier Proteins 010606 plant biology & botany Protein Binding |
| Zdroj: | International Journal of Molecular Sciences Volume 20 Issue 14 International Journal of Molecular Sciences, Vol 20, Iss 14, p 3388 (2019) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms20143388 |
| Popis: | Soybean mosaic virus (SMV) is one of the most prevalent and important pathogens of soybean, which produces 11 proteins, and the third protein, P3, was suggested to be involved in virus movement and replication, as well as host infection. During the virus infection, host proteins are essential in the virus cycle. However, there is no comprehensive report on the network of host proteins that interact with P3. Fifty-one interactors were identified by using the P3 protein as the bait against the SMV SC15 strain-challenged soybean cDNA library. These proteins were classified into five groups, including transport and protein transport-related proteins, defense and disease-related proteins, photosynthesis proteins, cellular metabolic proteins, and unknown proteins. Among these proteins, the protein defined as hypersensitive response-like lesion-inducing (HRLI) appeared multiple times and showed strong affinity with P3, which indicated its important role in SMV infection. Thus, it was chosen for further investigation. Phylogenetic classification showed that paralog proteins GmHRLI-1 and GmHRLI-2 clustered together and shared 90% homologous identity. Bimolecular fluorescence complementation (BiFC) assay was carried out to confirm the interaction, and fluorescence was detected at the cell periplasmic as well as at the nucleus. Subcellular localization showed that GmHRLI was localized to the cell periplasmic, while the co-localization of GmHRLI and P3 signals was also observed in the nucleus, suggesting that GmHRLI could interact with P3 and promoted the translation of P3 to the nucleus. Moreover, the gene expression of GmHRLI was abundant in the roots, leaves, and flowers, and could be induced by SMV infection, suggesting its involvement in SMV infection. Our results together lay the foundation to explore the mechanisms of P3 in the HR process and the HRLI protein function in SMV response. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |