STARCH-GEL ELECTROPHORESIS OF MOUSE PITUITARY GONADOTROPHINS
| Autor: | D. R. Lamond, H. M. Lloyd, B. M. Bindon, J. D. Meares |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
Electrophoresis
Male Ovulation endocrine system Pituitary gland medicine.medical_specialty Starch Endocrinology Diabetes and Metabolism media_common.quotation_subject Uterus Biology Chorionic Gonadotropin Mice Follicle-stimulating hormone chemistry.chemical_compound Endocrinology Internal medicine Methods medicine Animals media_common Body Weight Age Factors Organ Size Luteinizing Hormone Starch gel electrophoresis medicine.anatomical_structure chemistry Pituitary Gland Gonadotropins Pituitary Biological Assay Female Follicle Stimulating Hormone Luteinizing hormone Gels |
| Zdroj: | Journal of Endocrinology. 40:313-323 |
| ISSN: | 1479-6805 0022-0795 |
| Popis: | SUMMARY Extracts of mouse pituitary glands, prepared by homogenization and high-speed centrifugation, were separated into 16–17 fractions by preparative electrophoresis in starch gel, from which the proteins were then recovered by an electrophoretic method. Follicle-stimulating hormone (FSH), assayed by the uterine-weight augmentation method in the hypophysectomized mouse, was confined to 2–3 adjacent portions of the gel 1 cm. wide. Luteinizing hormone (LH) assayed by the rat ovarian ascorbic-acid depletion method was recovered from three discrete regions of the gel, well separated from FSH. The most active LH fraction was of slower mobility than the FSH. Assay for 'total' gonadotrophin, using normal mouse uterine weights, disclosed active material of slow mobility, separated from the FSH fractions. A series of experiments devoted to assays of FSH separated electrophoretically from pooled mouse pituitary glands demonstrated variations in pituitary content and concentration of FSH related to sex, age, pregnancy, lactation, dietary changes and light exposure which were consistent with results of previous studies of the mouse and of the rat. |
| Databáze: | OpenAIRE |
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