Genetic autonomy and low singlet oxygen yield support kleptoplast functionality in photosynthetic sea slugs
| Autor: | Esa Tyystjärvi, Mikko Antinluoma, Sergey Khorobrykh, Sven B. Gould, Vesa Havurinne, Maria Handrich |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
0106 biological sciences Chloroplasts Photoinhibition Physiology photosynthetic sea slugs Gastropoda PSII repair cycle Plant Science Biology Photosynthesis 01 natural sciences Vaucheria litorea 03 medical and health sciences Animals Plastids Plastid 030304 developmental biology reactive oxygen species 0303 health sciences Singlet Oxygen photoinhibition AcademicSubjects/SCI01210 fungi food and beverages biology.organism_classification Research Papers Sea slug Cell biology 030104 developmental biology Thylakoid Elysia chlorotica Spinach Kleptoplasty Photosynthesis and Metabolism 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany |
| DOI: | 10.1101/2021.02.02.429324 |
| Popis: | Vaucheria litorea plastids exhibit increases in the relative expression of key plastid maintenance genes upon isolation, and produce little singlet oxygen. These factors likely contribute to plastid longevity in kleptoplastic slugs. The kleptoplastic sea slug Elysia chlorotica consumes Vaucheria litorea, stealing its plastids, which then photosynthesize inside the animal cells for months. We investigated the properties of V. litorea plastids to understand how they withstand the rigors of photosynthesis in isolation. Transcription of specific genes in laboratory-isolated V. litorea plastids was monitored for 7 days. The involvement of plastid-encoded FtsH, a key plastid maintenance protease, in recovery from photoinhibition in V. litorea was estimated in cycloheximide-treated cells. In vitro comparison of V. litorea and spinach thylakoids was applied to investigate reactive oxygen species formation in V. litorea. In comparison to other tested genes, the transcripts of ftsH and translation elongation factor EF-Tu (tufA) decreased slowly in isolated V. litorea plastids. Higher levels of FtsH were also evident in cycloheximide-treated cells during recovery from photoinhibition. Charge recombination in PSII of V. litorea was found to be fine-tuned to produce only small quantities of singlet oxygen, and the plastids also contained reactive oxygen species-protective compounds. Our results support the view that the genetic characteristics of the plastids are crucial in creating a photosynthetic sea slug. The plastid’s autonomous repair machinery is likely enhanced by low singlet oxygen production and elevated expression of FtsH. |
| Databáze: | OpenAIRE |
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