Human melanoma brain metastases cell line MUG-Mel1, isolated clones and their detailed characterization
| Autor: | Ellen Heitzer, Sabrina Riedl, Diana Behrens, Dagmar Kolb-Lenz, Katharina Meditz, Carina Fischer, B. Ewa Snaar-Jagalska, Karoly Szuhai, Karl Kashofer, Birgit Lohberger, Arwin Groenewoud, Danielle de’Jong, Andreas Prokesch, Dagmar Zweytick, Johannes Haybaeck, Bernadette Liegl-Atzwanger, Helmut Schaider, Beate Rinner, Harald Koefeler |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Carcinogenesis Gene Dosage lcsh:Medicine Mice Nude Stem cell marker Gene dosage Article Metastasis 03 medical and health sciences Inhibitory Concentration 50 0302 clinical medicine In vivo Cell Line Tumor medicine Animals Humans lcsh:Science Zebrafish Melanoma Multidisciplinary biology Brain Neoplasms lcsh:R Lipidome Middle Aged medicine.disease biology.organism_classification Lipids Clone Cells 030104 developmental biology Cancer research Neoplastic Stem Cells lcsh:Q Female Peptides 030217 neurology & neurosurgery Brain metastasis |
| Zdroj: | Scientific Reports, 9, 4096 Scientific Reports, 9 Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-13 (2019) |
| Popis: | Melanoma is a leading cause of high mortality that frequently spreads to the brain and is associated with deterioration in quality and quantity of life. Treatment opportunities have been restricted until now and new therapy options are urgently required. Our focus was to reveal the potential heterogeneity of melanoma brain metastasis. We succeeded to establish a brain melanoma metastasis cell line, namely MUG-Mel1 and two resulting clones D5 and C8 by morphological variety, differences in lipidome, growth behavior, surface, and stem cell markers. Mutation analysis by next-generation sequencing, copy number profiling, and cytogenetics demonstrated the different genetic profile of MUG-Mel1 and clones. Tumorigenicity was unsuccessfully tested in various mouse systems and finally established in a zebra fish model. As innovative treatment option, with high potential to pass the blood-brain barrier a peptide isolated from lactoferricin was studied in potential toxicity. Brain metastases are a major clinical challenge, therefore the development of relevant in vitro and in vivo models derived from brain melanoma metastases provides valuable information about tumor biology and offers great potential to screen for new innovative therapies. |
| Databáze: | OpenAIRE |
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