In-silico Exploration of Channel Type and Efflux Silicon Transporters and Silicification Proteins in 80 Sequenced Viridiplantae Genomes
| Autor: | Xiao Lin, Rana Muhammad Atif, Muhammad Amjad Nawaz, Gyuhwa Chung, Alexander M. Zakharenko, Junghee Ham, Kirill S. Golokhvast, Ting-Fung Chan, Farrukh Azeem, Sangmi Sun, Faheem Shehzad Baloch |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Siliplant 1 In silico Aquaporin silica accumulation Viridiplantae Plant Science Chlorophyta channel type Si transporter 01 natural sciences Article NIPs 03 medical and health sciences influx transporter Gene Ecology Evolution Behavior and Systematics chemistry.chemical_classification Ecology biology Si efflux Botany Transporter biosilicification biology.organism_classification Cell biology Amino acid 030104 developmental biology chemistry QK1-989 Efflux 010606 plant biology & botany |
| Zdroj: | Plants Volume 9 Issue 11 Plants, Vol 9, Iss 1612, p 1612 (2020) |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants9111612 |
| Popis: | Silicon (Si) accumulation protects plants from biotic and abiotic stresses. It is transported and distributed within the plant body through a cooperative system of channel type (e.g., OsLsi1) and efflux (Lsi2s e.g., OsLsi2) Si transporters (SITs) that belong to Noduline-26 like intrinsic protein family of aquaporins and an uncharacterized anion transporter family, respectively. Si is deposited in plant tissues as phytoliths and the process is known as biosilicification but the knowledge about the proteins involved in this process is limited. In the present study, we explored channel type SITs and Lsi2s, and siliplant1 protein (Slp1) in 80 green plant species. We found 80 channel type SITs and 133 Lsi2s. The channel type SITs characterized by the presence of two NPA motifs, GSGR or STAR selectivity filter, and 108 amino acids between two NPA motifs were absent from Chlorophytes, while Streptophytes evolved two different types of channel type SITs with different selectivity filters. Both channel type SITs and Lsi2s evolved two types of gene structures each, however, Lsi2s are ancient and were also found in Chlorophyta. Homologs of Slp1 (225) were present in almost all Streptophytes regardless of their Si accumulation capacity. In Si accumulator plant species, the Slp1s were characterized by the presence of H, D-rich domain, P, K, E-rich domain, and P, T, Y-rich domain, while moderate Si accumulators lacked H, D-rich domain and P, T, Y-rich domains. The digital expression analysis and coexpression networks highlighted the role of channel type and Lsi2s, and how Slp1 homologs were ameliorating plants&rsquo ability to withstand different stresses by co-expressing with genes related to structural integrity and signaling. Together, the in-silico exploration made in this study increases our knowledge of the process of biosilicification in plants. |
| Databáze: | OpenAIRE |
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