Differential trafficking of live and dead Mycobacterium marinum organisms in macrophages
| Autor: | Kathleen George, Lucia P. Barker, P. L. C. Small, S. Falkow |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Immunology
Population Cathepsin D Vacuole Microbiology Cell Line Mycobacterium Mice Phagosomes Animals education Mycobacterium marinum Phagosome Latex beads Cathepsin education.field_of_study Microscopy Confocal biology Macrophages Biological Transport biology.organism_classification Cell biology Infectious Diseases Parasitology Research Article |
| Popis: | We characterized the Mycobacterium marinum phagosome by using a variety of endocytic markers to follow the path of the bacteria through a mouse macrophage cell line. Using a laser confocal microscope, we found that the majority of viable M. marinum cells were in nonacidic vacuoles that did not colocalize with the vacuolar proton ATPase (V-ATPase), the calcium-independent mannose-6-phosphate receptor (CI-M6PR), or cathepsin D. In contrast, heat-killed organisms and latex beads were in acidic vacuoles which contained the V-ATPase, the CI-M6PR, and cathepsin D. A population of vesicles that contained live M. marinum labeled with the lysosomal glycoprotein LAMP-1, but the percentage of vacuoles that labeled was lower than for heat-killed organisms or latex beads. When testing live and heat-killed Mycobacterium tuberculosis, we found levels of colocalization with LAMP- and cathepsin D comparable to those for the M. marinum isolate. We conclude that M. marinum, like M. tuberculosis, can circumvent the host endocytic pathway and reside in an intracellular compartment which is not acidic and does not fuse with lysosomes. In addition, we describe a system for sampling a large population of intracellular organisms by using a laser confocal microscope. |
| Databáze: | OpenAIRE |
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