Cloning and Expression of the Nucleoprotein of Peste des Petits Ruminants Virus in Baculovirus for Use in Serological Diagnosis
| Autor: | Jeremiah T. Saliki, Alaa A. El-Kholy, Tarek M. Ismail, Charles A. Mebus, Miles Yamanaka, Tilahun Yilma |
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| Rok vydání: | 1995 |
| Předmět: |
Genes
Viral Sequence analysis viruses Genetic Vectors Molecular Sequence Data Gene Expression Nigeria Enzyme-Linked Immunosorbent Assay Spodoptera Biology Antibodies Viral Virus Cell Line Peste-des-petits-ruminants virus law.invention Capsid law Virology Complementary DNA Animals Amino Acid Sequence Cloning Molecular Viral Structural Proteins Peste-des-Petits-Ruminants Base Sequence Viral Core Proteins Genetic Variation Sequence Analysis DNA Recombinant Proteins Nucleoprotein Molecular Weight Recombinant DNA biology.protein Antibody Baculoviridae Morbillivirus Infections |
| Zdroj: | Virology. 208:776-778 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.1995.1210 |
| Popis: | Peste des petits ruminants (PPR) is a viral disease of goats and sheep characterized by erosive stomatitis, enteriris, and pneumonia. The virus is a member of the family Paramyxoviridae and the genus Morbillivirus. The disease has high morbidity and mortality rates and has a substantial economic impact in developing countries. We have cloned and sequenced the cDNA of the nucleocapsid (N) gene of the Nigeria 75/1 strain of PPR virus (PPRV). A comparison of its nucleotide and deduced amino acid sequence with those of the N gene of the tissue culture-attenuated strain of PPRV was performed. A divergence of 8.9 and 5.0% was found at the nucleotide and amino acid level, respectively. A recombinant baculovirus that expresses the N protein in insect cells and larvae (Spodoptera frugiperda) was generated. The recombinant protein, characterized by Western blot analysis, was shown to have a molecular weight of 58 kDa and was recognized by anti-PPRV antibodies. The recombinant protein was used successfully as a coating antigen in an enzyme-linked immunosorbent assay for the serological diagnosis of PPRV. |
| Databáze: | OpenAIRE |
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