The effect of sampling procedures on the urate and lactate concentration in oral fluid

Autor: F. Di Francesco, Silvia Ghimenti, E. Bramanti, Massimo Onor, Tommaso Lomonaco, Francesca G. Bellagambi, Denise Biagini, Roger Fuoco
Rok vydání: 2018
Předmět:
Zdroj: Microchemical Journal
Microchemical journal
136 (2016): 255–262. doi:10.1016/j.microc.2017.02.032
info:cnr-pdr/source/autori:Lomonaco T.; Ghimenti S.; Biagini D.; Bramanti E.; Onor M.; Bellagambi F.G.; Fuoco R.; Di Francesco F./titolo:The effect of sampling procedures on the urate and lactate concentration in oral fluid/doi:10.1016%2Fj.microc.2017.02.032/rivista:Microchemical journal (Print)/anno:2016/pagina_da:255/pagina_a:262/intervallo_pagine:255–262/volume:136
ISSN: 0026-265X
DOI: 10.1016/j.microc.2017.02.032
Popis: This study was aimed at evaluating the influence of sampling procedure on the determination of uric acid and lactate in oral fluid. Samples of non-stimulated and stimulated oral fluid were collected from 22 healthy volunteers. Different frequencies of stimulation were obtained by moving a polyester swab within the mouth at 50, 100 and 150 min − 1 . Three oral fluid samples were consecutively collected from a subgroup of 5 volunteers at a constant stimulation (70 min − 1 ) and at a self-selected pace to evaluate reproducibility. The urate concentration in oral fluid decreased with the increase of the stimulation and oral fluid flow rate (r = − 0.98, p = 0.01). Also, the lactate concentration was much (p = 0.03, two tailed) lower in samples collected under a mild stimulation (50 min − 1 ) than in samples collected without stimulation. Nevertheless, it progressively increased at higher stimulations (100 and 150 min − 1 ). A transfer process mediated by membrane carriers (i.e. urate transporter and organic anion transporters) was hypothesized to explain these results. Finally, a reduced variability (relative standard deviation below 10%) of the urate concentration was obtained when oral fluid was sampled at constant stimulation (70 min − 1 ), but it increased remarkably (20–50%) in case of sampling at self-selected pace. Nevertheless, expressing the salivary excretion of urate as a function of time (μg min − 1 ), the variability of sampling procedure at self-selected pace was lower than 15%.
Databáze: OpenAIRE
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