Immuno‐Quantitative Polymerase Chain Reaction for Detection and Quantitation of Prion Protein
| Autor: | Stéphanie Gofflot, Dominique Quatpers, Ernst Heinen, Benaïssa Elmoualij, Willy Zorzi, Laurence Melen-Lamalle, Emmanuelle Vanopdenbosch, Stéphane Roels, Jacques Grassi, Danièle Zorzi |
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| Rok vydání: | 2004 |
| Předmět: |
Brain Chemistry
Prions Clinical Biochemistry Immunology Antibodies Monoclonal Enzyme-Linked Immunosorbent Assay Biology Polymerase Chain Reaction Sensitivity and Specificity Molecular biology Reverse transcription polymerase chain reaction Medical Laboratory Technology Real-time polymerase chain reaction Bovine brain Cricetinae Primer dimer Animals Humans Immunology and Allergy Cattle Prion protein Antibody detection |
| Zdroj: | Journal of Immunoassay and Immunochemistry. 25:241-258 |
| ISSN: | 1532-4230 1532-1819 |
| DOI: | 10.1081/ias-200028044 |
| Popis: | Immuno‐polymerase chain reaction (PCR) is an extremely sensitive detection method, combining the specificity of antibody detection and the sensitivity of PCR. We have developed an immuno‐quantitative PCR (iqPCR), exploiting real‐time PCR technology, in order to improve this immuno‐detection method and make it quantitative. To illustrate the advantages of iqPCR, we have compared it with a conventional enzyme linked immuno sorbent assay (ELISA) technique in experiments aimed at detecting the cellular and the resistant form of prion protein in bovine brain extract. The iqPCR technique proved to be more sensitive than ELISA, so it could be a technique of choice for the diagnosis of infected animals both at an ante mortem and post‐mortem stage. |
| Databáze: | OpenAIRE |
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