Simultaneous determination of metformin and vildagliptin in human plasma by a HILIC–MS/MS method
| Autor: | Thais Martins Guimarães de Francisco, Ana Carolina Gimenez, Flávia Lada Degaut Pontes, Roberto Pontarolo, Rômulo Pereira Ribeiro, João Cleverson Gasparetto |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Pyrrolidines Formic acid Clinical Biochemistry Adamantane Biochemistry Analytical Chemistry Matrix (chemical analysis) chemistry.chemical_compound Drug Stability Limit of Detection Tandem Mass Spectrometry Nitriles medicine Ammonium formate Humans Vildagliptin Detection limit Chromatography Hydrophilic interaction chromatography Reproducibility of Results Cell Biology General Medicine Middle Aged Metformin chemistry Human plasma Linear Models Female Hydrophobic and Hydrophilic Interactions Chromatography Liquid medicine.drug |
| Zdroj: | Journal of Chromatography B. 965:133-141 |
| ISSN: | 1570-0232 |
| Popis: | The objective of this work was to develop and validate a HILIC-MS/MS method for the simultaneous determination of metformin and vildagliptin in human plasma. Chromatographic separation was achieved using an Atlantis HILIC Silica 150-mm × 2.1-mm, 3-μm particle size column maintained at 40°C. The isocratic mobile phase consisted of 20% water and 80% acetonitrile/water solution 95:5 (v/v), containing both 0.1% formic acid and 3mM ammonium formate. The flow rate was maintained at 400 μL min(-1). Data from validation studies demonstrated that the new method is highly selective, sensitive (limits of detection |
| Databáze: | OpenAIRE |
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