Detection of 14-3-3ζ in cerebrospinal fluid following experimentally evoked seizures
| Autor: | Niamh C. Murphy, Akitaka Yamamoto, David C. Henshall |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Kainic acid Pathology medicine.medical_specialty Health Toxicology and Mutagenesis Clinical Biochemistry Central nervous system Nerve Tissue Proteins Neurological disorder Hippocampus Biochemistry Epileptogenesis Rats Sprague-Dawley chemistry.chemical_compound Epilepsy Cerebrospinal fluid Seizures Convulsion medicine Animals Kainic Acid biology business.industry medicine.disease Rats medicine.anatomical_structure 14-3-3 Proteins nervous system chemistry biology.protein medicine.symptom NeuN business Biomarkers |
| Zdroj: | Biomarkers. 13:377-384 |
| ISSN: | 1366-5804 1354-750X |
| DOI: | 10.1080/13547500802027971 |
| Popis: | Surrogate and peripheral (bio)markers of neuronal injury may be of value in assessing effects of seizures on the brain or epilepsy development following trauma. The presence of 14-3-3 isoforms in cerebrospinal fluid (CSF) is a diagnostic indicator of Creutzfeldt-Jakob disease but these proteins may also be present following acute neurological insults. Here, we examined neuronal and 14-3-3 proteins in CSF from rats after seizures. Seizures induced by intra-amygdala microinjection of 0.1 microg kainic acid (KA) caused damage which was mainly restricted to the ipsilateral CA3 subfield of the hippocampus. 14-3-3zeta was detected at significant levels in CSF sampled 4 h after seizures compared with near absence in control CSF. Neuron-specific nuclear protein (NeuN) was also elevated in CSF in seizure rats. CSF 14-3-3zeta levels were significantly lower in rats treated with 0.01 microg KA. These data suggest the presence of 14-3-3zeta within CSF may be a biomarker of acute seizure damage. |
| Databáze: | OpenAIRE |
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