Anti-inflammatory and antinociceptive effects of phonophoresis in animal models: a randomized experimental study

Autor: G.M. Pires, M.R. Silva, N.B. Pinto, G.S.B. Viana, L.C.P. Cardoso, M.J.P. Lopes, M.E.P. Nobre, L.M.R. Rodrigues
Rok vydání: 2019
Předmět:
Male
Nociception
0301 basic medicine
Physiology
Administration
Topical
Ultrasonic Therapy
Anti-Inflammatory Agents
Phonophoresis
Pharmacology
Biochemistry
Random Allocation
chemistry.chemical_compound
0302 clinical medicine
Edema
Prospective Studies
General Pharmacology
Toxicology and Pharmaceutics
lcsh:QH301-705.5
Physiotherapy
Research Articles
lcsh:R5-920
Analgesics
General Neuroscience
Anti-Inflammatory Agents
Non-Steroidal
General Medicine
Ultrasonic therapy
030220 oncology & carcinogenesis
Tumor necrosis factor alpha
medicine.symptom
lcsh:Medicine (General)
medicine.drug
Diclofenac
medicine.drug_class
Immunology
Biophysics
Ocean Engineering
Inflammation
Anti-inflammatory
03 medical and health sciences
medicine
Animals
Rats
Wistar
Tumor Necrosis Factor-alpha
business.industry
Therapeutic effect
Cell Biology
Rats
Carrageenan
Disease Models
Animal
030104 developmental biology
lcsh:Biology (General)
chemistry
business
Zdroj: Brazilian Journal of Medical and Biological Research, Volume: 52, Issue: 2, Article number: e7773, Published: 24 JAN 2019
Brazilian Journal of Medical and Biological Research v.52 n.2 2019
Brazilian Journal of Medical and Biological Research
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Brazilian Journal of Medical and Biological Research, Vol 52, Iss 2 (2019)
ISSN: 1414-431X
0100-879X
Popis: The aim of this study was to evaluate the therapeutic effects of ultrasound (US)-mediated phonophoresis alone or in association with diclofenac diethylammonium (DCF) administered topically in animal models of inflammation. A pre-clinical, prospective, and randomized experimental study of quantitative and qualitative nature was carried out. Phonophoresis was performed using a therapeutic ultrasound apparatus in two distinct models of acute inflammation. Edema was induced by an intraplantar injection of carrageenan and measured by plethysmography. The Hargreaves test was used to evaluate the antinociceptive activity and investigate the action of phonophoresis on tumor necrosis factor (TNF)-α production. A histological analysis with hematoxylin-eosin was used to evaluate tissue repair, and the expression of COX-2 was determined by immunohistochemical analysis. At the peak of inflammatory activity (3 h), treatment with US, US+DCF, and DCF significantly reduced edema formation compared to the control group. Treatment with US+DCF was more effective than treatment with US alone at both analyzed times. In the analysis of the antinociceptive activity, the treatments significantly increased the latency time in response to the thermal stimulus. Histopathological analysis revealed a reduction of the inflammatory infiltrates and immunohistochemistry demonstrated that the association was effective in reducing COX-2 expression compared to the control group. The association of DCF with US produced anti-inflammatory and antinociceptive effects in rat models of inflammation, which may be associated with inhibition of COX-2 and TNF-α production.
Databáze: OpenAIRE
Externí odkaz: