Magnesium promotes the viability and induces differentiation of neural stem cells both in vitro and in vivo
| Autor: | Xi-Jiao Yu, Hong-Mei Liu, Linwang Su, Lande Xue, Jian-Li Xie, Huan Jiang, Chao Wu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell Survival chemistry.chemical_element Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Neural Stem Cells Parkinsonian Disorders In vivo Animals Magnesium Maze Learning Cells Cultured Nootropic Agents reproductive and urinary physiology Neurons Ideal (set theory) Cell Differentiation General Medicine In vitro Neural stem cell nervous system diseases Cell biology Neuroprotective Agents 030104 developmental biology nervous system Neurology chemistry Multipotent Stem Cell Neurology (clinical) biological phenomena cell phenomena and immunity Neuroglia 030217 neurology & neurosurgery |
| Zdroj: | Neurological Research. 41:208-215 |
| ISSN: | 1743-1328 0161-6412 |
| DOI: | 10.1080/01616412.2018.1544400 |
| Popis: | Neural stem cells (NSCs) are multipotent stem cells that generating various neural cells, including neurons, astrocytes and oligodendrocytes. This showed that NSCs is an ideal candidate in the application of neural disease treatment. In the current study, we established a simple and efficient method to promote the viability and induce the differentiation of NSCs by stimulating with magnesium.The proliferation and differentiation of NSCs was determined by MTT assay and immunostaining. The behavior alteration was measured by rotorod test and Morris water maze.Magnesium enhanced proliferation in NSCs. The ratio of Nestin+, Ki67+ and GFAP+ progenitor cells was increased in the presence of magnesium. Besides, magnesium induced the glial differentiation instead of neuronal differentiation in NSCs. By contrast, transplantation of Mg2+-treated NSCs in vivo generated more neurons. In established PD models, transplantation of Mg2+-treated NSCs could improve the symptoms and recover the memory.We established a simple and efficient way to promote the proliferation and induce the differentiation of NSCs. More importantly, this may also facilitate to develop a new method to neural disorder treatment. |
| Databáze: | OpenAIRE |
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