Natural antibody–complement dependent neutralization of bovine herpesvirus 4 by human serum
| Autor: | Yannick Nizet, Christophe Bona, Bérénice Costes, Cédric Delforge, Alain Vanderplasschen, Laurent Gillet, Sieberth Do Nascimento Brito, Pierre Gianello, Pierre Drion, Nicolas Markine-Goriaynoff, Bénédicte Machiels |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
medicine.drug_class
Immunology Biology Antibodies Viral Monoclonal antibody medicine.disease_cause Microbiology Epitope Virus Herpesviridae Neutralization Cell Line Neutralization Tests Chlorocebus aethiops medicine Animals Humans Complement Pathway Classical Complement Activation Vero Cells Immune Sera Virology Immunity Innate In vitro Herpesvirus 4 Bovine Infectious Diseases Immunoglobulin M Bovine herpesvirus 4 Immunoglobulin G biology.protein Cattle Antibody Trisaccharides |
| Zdroj: | Microbes and Infection. 9:1530-1537 |
| ISSN: | 1286-4579 |
| DOI: | 10.1016/j.micinf.2007.08.007 |
| Popis: | In contrast to most gammaherpesviruses, Bovine herpesvirus 4 (BoHV-4) has a broad range of host species both in vitro and in vivo. Several in vitro studies demonstrated that some human cell lines are sensitive or even permissive to BoHV-4. These observations led to the hypothesis that cross-species transmission of BoHV-4 could lead to human infections. In the present study, we investigate the sensitivity of BoHV-4 to neutralization by naïve human sera in order to determine if humans exhibit innate anti-viral activities against this virus. Our results demonstrate that human sera from naïve individuals, in contrast to the sera of naïve subjects from various animal species, neutralize BoHV-4 efficiently. A series of complementary experiments were performed to unravel the mechanism(s) of this neutralization. The data obtained in this study demonstrates that human serum neutralizes BoHV-4 in a complement dependent manner activated by natural antibodies raised against the Galalpha1-3Galbeta1-4GlcNAc-R epitope expressed by bovine cells. |
| Databáze: | OpenAIRE |
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