Induction of cytokine expression in the brain macrophages/amoeboid microglia of the fetal rat exposed to a teratogen
| Autor: | Ling Ea, Dheen St, AJ Hao |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
Time Factors medicine.medical_treatment In situ hybridization Biology Avian Proteins Rats Sprague-Dawley Fetus Antigens CD Antigens Neoplasm Cell Movement Pregnancy Transforming Growth Factor beta Internal medicine Lectins medicine Macrophage Animals RNA Messenger Cyclophosphamide Cellular localization Membrane Glycoproteins Microglia Interleukin-6 Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha General Neuroscience Macrophages Brain Gene Expression Regulation Developmental Blood Proteins Molecular biology Rats medicine.anatomical_structure Endocrinology Cytokine Teratogens Prenatal Exposure Delayed Effects Antigens Surface Basigin Immunohistochemistry Neuroglia Cytokines Tumor necrosis factor alpha Female Interleukin-1 Mutagens |
| Zdroj: | Neuroreport. 12(7) |
| ISSN: | 0959-4965 |
| Popis: | Using in situ hybridization, RT-PCR, lectin histochemistry and immunohistochemistry, this study examined the time course expression and cellular localization of various cytokines including tumor necrosis factor-alpha (TNF-alpha), interleukins (IL-1, IL-6) and transforming growth factor-beta (TGF-beta) in fetal rat brain after a maternal injection of the teratogen cyclophosphamide (CP). Eight hours after CP injection, there was a marked increase in brain macrophages (BM)/amoeboid microglia (AM) in different areas of the fetal brain as determined by lectin histochemistry. Concomitant to this was the induction in mRNA level of TNF-alpha, which was progressively increased with time. TGF-beta mRNA was undetectable until 24 h had elapsed. Expression of IL-1 and IL-6 was undetectable at all stages. In situ hybridization combined with immunohistochemistry has shown the localization of TNF-alpha in BM/AM and neurons. Present results suggest that both TNF-alpha and TGF-beta are involved in the progression of neural damage in the fetal brain induced by the teratogen. |
| Databáze: | OpenAIRE |
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