Protocol and application of basal erythrocyte transketolase activity to improve assessment of thiamine status
| Autor: | Kerry S. Jones, Damon A. Parkington, Megan W. Bourassa, Carla Cerami, Albert Koulman |
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| Přispěvatelé: | Jones, Kerry S [0000-0002-7380-9797], Apollo - University of Cambridge Repository |
| Rok vydání: | 2023 |
| Předmět: |
vitamin B1
Erythrocytes General Neuroscience Thiamine Deficiency women of reproductive age beriberi General Biochemistry Genetics and Molecular Biology Hemoglobins History and Philosophy of Science micronutrient deficiency Humans Female Thiamine Transketolase ORIGINAL ARTICLES Biomarkers ORIGINAL ARTICLE |
| Popis: | Thiamine (vitamin B1) is an essential micronutrient required as a cofactor in many metabolic processes. Clinical symptoms of thiamine deficiency are poorly defined, hence biomarkers of thiamine status are important. The erythrocyte transketolase activity coefficient (ETKac) is a sensitive measure of thiamine status, but its interpretation may be confounded where the availability of the transketolase enzyme is limited. Basal ETK activity per gram of hemoglobin provides a complementary biomarker of thiamine status; however, its measurement and calculation are poorly described. Here, we describe in detail the assessment of basal ETK activity, including the calculation of path length in microplates and the molar absorption coefficient of NADH specific to the assay, and the measurement of hemoglobin in sample hemolysates. To illustrate the application of the methods, we present ETKac and basal ETK activity from women in The Gambia and UK. In conclusion, we present a clear protocol for the measurement of basal ETK activity that will permit the harmonization of methods to improve replication between laboratories. This work was funded by the NIHR Cambridge Biomedical Research Centre (IS-BRC-1215-20014). The views expressed are those of the authors and not necessarily those of the NHS, the NIHR or the Department of Health and Social Care. |
| Databáze: | OpenAIRE |
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