First in patient assessment of brain tumor infiltrative margins using simultaneous time-resolved measurements of 5-ALA-induced PpIX fluorescence and tissue autofluorescence
| Autor: | Alba Alfonso-García, Xiangnan Zhou, Julien Bec, Silvia N. Anbunesan, Farzad Fereidouni, Lee-Way Jin, Han S. Lee, Orin Bloch, Laura Marcu |
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| Rok vydání: | 2021 |
| Předmět: |
5-ALA-induced PpIX fluorescence
Letter Biomedical Engineering Protoporphyrins Bioengineering Optical Physics Fluorescence Biomaterials Rare Diseases Clinical Research Opthalmology and Optometry Humans Cancer Photosensitizing Agents Brain Neoplasms Neurosciences glioblastoma Margins of Excision Optics Aminolevulinic Acid nicotinamide adenine (phosphate) dinucleotide JBO Letters Atomic and Molecular Physics and Optics fluorescence lifetime imaging Electronic Optical and Magnetic Materials Brain Disorders Brain Cancer brain tumor |
| Zdroj: | Journal of Biomedical Optics Journal of biomedical optics, vol 27, iss 2 |
| ISSN: | 1560-2281 |
| Popis: | Significance5-aminolevulinic acid (5-ALA)-induced protoporphyrin IX (PpIX) fluorescence is currently used for image-guided glioma resection. Typically, this widefield imaging method highlights the bulk of high-grade gliomas, but it underperforms at the infiltrating edge where PpIX fluorescence is not visible to the eyes. Fluorescence lifetime imaging (FLIm) has the potential to detect PpIX fluorescence below the visible detection threshold. Moreover, simultaneous acquisition of time-resolved nicotinamide adenine (phosphate) dinucleotide [NAD(P)H] fluorescence may provide metabolic information from the tumor environment to further improve overall tumor detection.AimWe investigate the ability of pulse sampling, fiber-based FLIm to simultaneously image PpIX and NAD(P)H fluorescence of glioma infiltrative margins in patients.ApproachA mesoscopic fiber-based point-scanning FLIm device (355nm pulses) was used to simultaneously resolve the fluorescence decay of PpIX (629/53nm) and NAD(P)H (470/28nm). The FLIm device enabled data acquisition at room light and rapid ( |
| Databáze: | OpenAIRE |
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