Polyglutamine-containing microglia leads to disturbed differentiation and neurite retraction of neuron-like cells
| Autor: | Yohei Kakuta, Ryuji Owada, Saaya Awata, Hiroyasu Kanetaka, Kazutomo Suzue, Kazuhiro Nakamura |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Programmed cell death Neurite Cellular differentiation Neuronal death Molecular neuroscience 03 medical and health sciences 0302 clinical medicine Cell differentiation medicine lcsh:Social sciences (General) lcsh:Science (General) Multidisciplinary Microglia Chemistry Cell biology Neuroanatomy 030104 developmental biology medicine.anatomical_structure nervous system Apoptosis Cell culture lcsh:H1-99 Neuron Polyglutamine 030217 neurology & neurosurgery Research Article lcsh:Q1-390 |
| Zdroj: | Heliyon, Vol 6, Iss 9, Pp e04851-(2020) Heliyon |
| ISSN: | 2405-8440 |
| Popis: | Expanded polyglutamine-containing proteins in neurons intrinsically contributes to neuronal dysfunctions and neuronal cell death in polyglutamine (polyQ) diseases. In addition, an expanded polyQ-containing protein in microglia also leads to apoptosis of neurons. However, detailed morphological analysis of neurons exposed to conditioned medium (CM) derived from polyQ-containing microglia has not been essentially carried out. Here, we introduced aggregated peptide with 69 glutamine repeat (69Q) into BV2 microglial cells. The 69Q-containing BV2 cells showed shorter branches. The CM from 69Q-containing microglia (69Q-CM) induced neurite retraction and fewer number of branch point of neurites of differentiated PC12 cells. Likewise, the 69Q-CM induces disturbed differentiation of PC12 cells with shorter total length of neurites and fewer number of branch point of neurites. Thus, the factor(s) released from polyQ-containing microglia affect both differentiation and degeneration of neuron-like cells. Neurite, Polyglutamine, Neuronal death, Molecular neuroscience, Neuroanatomy, Cell culture, Cell differentiation |
| Databáze: | OpenAIRE |
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