670nm photobiomodulation modulates bioenergetics and oxidative stress, in rat Müller cells challenged with high glucose
| Autor: | Alexandria E. Hall, Janis T. Eells, Elizabeth S. Liedhegner, Gopika SenthilKumar, Betsy Abroe, Hannah J. Nonarath |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Light
Mitochondrion Pharmacology medicine.disease_cause Biochemistry chemistry.chemical_compound Cell Signaling Medicine and Health Sciences Enzyme assays Public and Occupational Health Colorimetric assays Bioassays and physiological analysis Immune Response Energy-Producing Organelles Cells Cultured chemistry.chemical_classification Multidisciplinary MTT assay Protease Inhibitor Therapy Chemistry Organic Compounds Physics Electromagnetic Radiation Monosaccharides Vaccination and Immunization Mitochondria Vascular endothelial growth factor Physical Sciences Medicine medicine.symptom Cellular Structures and Organelles Intracellular Research Article Signal Transduction Infrared Rays Science Immunology Ependymoglial Cells Carbohydrates Antiretroviral Therapy Inflammation Bioenergetics Glucose Signaling Signs and Symptoms Antiviral Therapy In vivo medicine Animals Reactive oxygen species Organic Chemistry Chemical Compounds Biology and Life Sciences Cell Biology In vitro Rats Research and analysis methods Oxidative Stress Glucose Sweetening Agents Biochemical analysis Preventive Medicine Clinical Medicine Energy Metabolism Reactive Oxygen Species Oxidative stress |
| Zdroj: | PLoS ONE PLoS ONE, Vol 16, Iss 12, p e0260968 (2021) |
| ISSN: | 1932-6203 |
| Popis: | Diabetic retinopathy (DR), the most common complication of diabetes mellitus, is associated with oxidative stress, nuclear factor-κB (NFκB) activation, and excess production of vascular endothelial growth factor (VEGF) and intracellular adhesion molecule-1 (ICAM-1). Muller glial cells, spanning the entirety of the retina, are involved in DR inflammation. Mitigation of DR pathology currently occurs via invasive, frequently ineffective therapies which can cause adverse effects. The application of far-red to near-infrared (NIR) light (630-1000nm) reduces oxidative stress and inflammation in vitro and in vivo. Thus, we hypothesize that 670nm light treatment will diminish oxidative stress preventing downstream inflammatory mechanisms associated with DR initiated by Muller cells. In this study, we used an in vitro model system of rat Müller glial cells grown under normal (5 mM) or high (25 mM) glucose conditions and treated with a 670 nm light emitting diode array (LED) (4.5 J/cm2) or no light (sham) daily. We report that a single 670 nm light treatment diminished reactive oxygen species (ROS) production and preserved mitochondrial integrity in this in vitro model of early DR. Furthermore, treatment for 3 days in culture reduced NFκB activity to levels observed in normal glucose and prevented the subsequent increase in ICAM-1. The ability of 670nm light treatment to prevent early molecular changes in this in vitro high glucose model system suggests light treatment could mitigate early deleterious effects modulating inflammatory signaling and diminishing oxidative stress. |
| Databáze: | OpenAIRE |
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