IL-8 expression and its possible relationship with estrogen-receptor-negative status of breast cancer cells
| Autor: | Jean-Paul Brouillet, Anne Licznar, Annick Lucas, Gwendal Lazennec, Françoise Vignon, Matthieu Lacroix, Corine Chauveau, Ariane Freund |
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| Přispěvatelé: | Endocrinologie moléculaire et cellulaire des cancers, Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Biologie Cellulaire et Hormonale, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Hôpital Arnaud de Villeneuve, Lazennec, Gwendal |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
CA15-3
Cancer Research Estrogen receptor MESH: Amino Acid Sequence MESH: Base Sequence 0302 clinical medicine Tumor Cells Cultured 0303 health sciences Carcinoma Ductal Breast Interleukin-18 MESH: Gene Expression Regulation Neoplastic MESH: Receptors Estro Gene Expression Regulation Neoplastic Receptors Estrogen 030220 oncology & carcinogenesis MESH: Cell Division Female Breast disease MESH: Interleukin-18 Cell Division medicine.medical_specialty Carcinogenicity Tests Molecular Sequence Data CA 15-3 Breast Neoplasms [SDV.CAN]Life Sciences [q-bio]/Cancer Biology Article 03 medical and health sciences Breast cancer [SDV.CAN] Life Sciences [q-bio]/Cancer Internal medicine Genetics medicine Humans Neoplasm Invasiveness Amino Acid Sequence Interleukin 8 Molecular Biology 030304 developmental biology MESH: Molecular Sequence Data MESH: Humans Base Sequence Cancer MESH: Neoplasm Invasiveness medicine.disease MESH: Carcinoma Ductal Breast Endocrinology MESH: Carcinogenicity Tests Cancer cell Cancer research MESH: Female MESH: Breast Neoplasms |
| Zdroj: | Oncogene Oncogene, Nature Publishing Group, 2003, 22 (2), pp.256-65. ⟨10.1038/sj.onc.1206113⟩ |
| ISSN: | 0950-9232 1476-5594 |
| DOI: | 10.1038/sj.onc.1206113⟩ |
| Popis: | Estrogen-receptor (ER) status is an important parameter in breast cancer management as ER-positive breast cancers have a better prognosis than ER-negative tumors. This difference comes essentially from the lower aggressiveness and invasiveness of ER-positive tumors. Here, we demonstrate, that interleukin-8 (IL-8) was clearly overexpressed in most ER-negative breast, ovary cell lines and breast tumor samples tested, whereas no significant IL-8 level could be detected in ER-positive breast or ovarian cell lines. We have also cloned human IL-8 from ER-negative MDA-MB-231 cells, and we show that IL-8 produced by breast cancer cells is identical to monocyte-derived IL-8. Interestingly, the invasion potential of ER-negative breast cancer cells is associated at least in part with expression of IL-8, but not with IL-8 receptor levels. Moreover, IL-8 increases the invasiveness of ER-positive breast cancer cells by two fold, thus confirming the invasion-promoting role of IL-8. On the other hand, exogenous expression of estrogen receptors in ER-negative cells led to a decrease of IL-8 levels. In summary, our data show that IL-8 expression is negatively linked to ER status of breast and ovarian cancer cells. We also support the idea that IL-8 expression is associated with a higher invasiveness potential of cancer cells in vitro, which suggests that IL-8 could be a novel marker of tumor aggressiveness. |
| Databáze: | OpenAIRE |
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