Interplay of Cell Shape and Division Orientation Promotes Robust Morphogenesis of Developing Epithelia
| Autor: | Kishore R. Mosaliganti, Tom W. Hiscock, Wenzhe Ma, Nicolas Rannou, Fengzhu Xiong, Nikolaus D. Obholzer, Sean G. Megason, Lydie Souhait, Ian A. Swinburne, Kenneth A. Brakke, Andrea R. Tentner, Arnaud Gelas |
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| Jazyk: | angličtina |
| Předmět: |
0303 health sciences
biology Cell division Biochemistry Genetics and Molecular Biology(all) Cell Morphogenesis Anatomy Division (mathematics) biology.organism_classification Embryonic stem cell General Biochemistry Genetics and Molecular Biology Flattening Cell biology Coupling (electronics) 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure medicine Zebrafish 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Cell. (2):415-427 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/j.cell.2014.09.007 |
| Popis: | SummaryEpithelial cells acquire functionally important shapes (e.g., squamous, cuboidal, columnar) during development. Here, we combine theory, quantitative imaging, and perturbations to analyze how tissue geometry, cell divisions, and mechanics interact to shape the presumptive enveloping layer (pre-EVL) on the zebrafish embryonic surface. We find that, under geometrical constraints, pre-EVL flattening is regulated by surface cell number changes following differentially oriented cell divisions. The division pattern is, in turn, determined by the cell shape distribution, which forms under geometrical constraints by cell-cell mechanical coupling. An integrated mathematical model of this shape-division feedback loop recapitulates empirical observations. Surprisingly, the model predicts that cell shape is robust to changes of tissue surface area, cell volume, and cell number, which we confirm in vivo. Further simulations and perturbations suggest the parameter linking cell shape and division orientation contributes to epithelial diversity. Together, our work identifies an evolvable design logic that enables robust cell-level regulation of tissue-level development. |
| Databáze: | OpenAIRE |
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