Spot the Difference-Development of a Syndrome Based Protein Microarray for Specific Serological Detection of Multiple Flavivirus Infections in Travelers
| Autor: | Cleton, NB, Godeke, G-J, Reimerink, J, Beersma, MF, Doorn, HRV, Franco, L, Goeijenbier, M, Jimenez-Clavero, MA, Johnson, BW, Niedrig, M, Papa, A, Sambri, V, Tami, A, Velasco-Salas, ZI, Koopmans, MPG, Reusken, CBEM, Day, NP |
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| Přispěvatelé: | Microbes in Health and Disease (MHD), Cleton, Nb, Godeke, Gj, Reimerink, J, Beersma, Mf, Doorn, Hr, Franco, L, Goeijenbier, M, Jimenez-Clavero, Ma, Johnson, Bw, Niedrig, M, Papa, A, Sambri, V, Tami, A, Velasco-Salas, Zi, Koopmans, Mp, Reusken, Cb, Virology, Day, N |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
SERUM ANTIBODIES viruses NS1 Dengue virus Viral Nonstructural Proteins NONSTRUCTURAL GLYCOPROTEIN medicine.disease_cause Antibodies Viral WEST-NILE-VIRUS Serology Epitopes Multiplex Flavivirus Infections 0303 health sciences Travel biology lcsh:Public aspects of medicine virus diseases Middle Aged 3. Good health Vaccination Flavivirus Infectious Diseases CROSS-REACTIVITY Protein microarray Female Research Article lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Protein Array Analysis Viremia Cross Reactions DIAGNOSIS 03 medical and health sciences SDG 3 - Good Health and Well-being medicine Humans Flavivirus serology microarrays antibody response DENGUE VIRUS 030304 developmental biology LINKED-IMMUNOSORBENT-ASSAY 030306 microbiology Public Health Environmental and Occupational Health lcsh:RA1-1270 biochemical phenomena metabolism and nutrition biology.organism_classification medicine.disease Virology ENCEPHALITIS-VIRUS Immunology IMMUNOGLOBULIN-M |
| Zdroj: | PLoS Neglected Tropical Diseases, 9(3):e0003580. PUBLIC LIBRARY SCIENCE PLoS Neglected Tropical Diseases, Vol 9, Iss 3, p e0003580 (2015) PLoS Neglected Tropical Diseases Repisalud Instituto de Salud Carlos III (ISCIII) Repositorio de Resultados de Investigación del INIA INIA: Repositorio de Resultados de Investigación del INIA PLoS Neglected Tropical Diseases (print), 9(3). Public Library of Science Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA |
| ISSN: | 1935-2735 |
| Popis: | Background The family Flaviviridae, genus Flavivirus, holds many of the world’s most prevalent arboviral diseases that are also considered the most important travel related arboviral infections. In most cases, flavivirus diagnosis in travelers is primarily based on serology as viremia is often low and typically has already been reduced to undetectable levels when symptoms set in and patients seek medical attention. Serological differentiation between flaviviruses and the false-positive results caused by vaccination and cross-reactivity among the different species, are problematic for surveillance and diagnostics of flaviviruses. Their partially overlapping geographic distribution and symptoms, combined with increase in travel, and preexisting antibodies due to flavivirus vaccinations, expand the need for rapid and reliable multiplex diagnostic tests to supplement currently used methods. Goal We describe the development of a multiplex serological protein microarray using recombinant NS1 proteins for detection of medically important viruses within the genus Flavivirus. Sera from clinical flavivirus patients were used for primary development of the protein microarray. Results Results show a high IgG and IgM sensitivity and specificity for individual NS1 antigens, and limited cross reactivity, even within serocomplexes. In addition, the serology based on this array allows for discrimination between infection and vaccination response for JEV vaccine, and no cross-reactivity with TBEV and YFV vaccine induced antibodies when testing for antibodies to other flaviviruses. Conclusion Based on these data, multiplex NS1-based protein microarray is a promising tool for surveillance and diagnosis of flaviviruses. Author Summary The number of international travelers has increased dramatically in recent decades. This has contributed to the increase in infectious diseases in travelers which are not present in their countries of origin and so may cause a threat to the public health. Viruses transmitted by biting insects (vector-borne viruses) are an important group within these travel-related diseases. They are found across the world and can cause debilitating and life-threatening symptoms, like inflammation of the brain or excessive bleeding. Many of these diseases are difficult to distinguish from each other. They cause comparable symptoms and are genetically closely related. Testing for long lists of diseases is time consuming and expensive. Here we develop a novel testing tool that allows doctors and researchers to test for multiple viruses with just one test. The method, which uses a specific part of the virus that makes distinguishing between infections with these closely related viruses possible, requires only one drop of blood. This allows us to test for multiple viruses simultaneously with the same amount of blood previously used to test for only one virus, while distinguishing between genetically closely related viruses. |
| Databáze: | OpenAIRE |
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