PHB Biosynthesis Counteracts Redox Stress in Herbaspirillum seropedicae
| Autor: | Glaucio Valdameri, Michelle Zibetti Tadra Sfeir, Ray Dixon, Marcelo Müller-Santos, Guilherme L. Sassaki, Emanuel Maltempi de Souza, Maria B. R. Steffens, Marcelo Bueno Batista, Luis Paulo Silveira Alves, Fábio O. Pedrosa, Cícero S. Teixeira |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Cytochrome Fnr 030106 microbiology Mutant lcsh:QR1-502 macromolecular substances Herbaspirillum seropedicae Microbiology Redox lcsh:Microbiology Polyhydroxyalkanoates redox regulation transcriptomics 03 medical and health sciences FNR regulon bacterial signal transduction biology Chemistry polyhydroxyalkanoates technology industry and agriculture biology.organism_classification Electron transport chain Cell biology 030104 developmental biology biology.protein lipids (amino acids peptides and proteins) Bacteria |
| Zdroj: | Frontiers in Microbiology, Vol 9 (2018) |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2018.00472 |
| Popis: | The ability of bacteria to produce polyhydroxyalkanoates such as poly(3-hydroxybutyrate) (PHB) enables provision of a carbon storage molecule that can be mobilized under demanding physiological conditions. However, the precise function of PHB in cellular metabolism has not been clearly defined. In order to determine the impact of PHB production on global physiology, we have characterized the properties of a ΔphaC1 mutant strain of the diazotrophic bacterium Herbaspirillum seropedicae. The absence of PHB in the mutant strain not only perturbs redox balance and increases oxidative stress, but also influences the activity of the redox-sensing Fnr transcription regulators, resulting in significant changes in expression of the cytochrome c-branch of the electron transport chain. The synthesis of PHB is itself dependent on the Fnr1 and Fnr3 proteins resulting in a cyclic dependency that couples synthesis of PHB with redox regulation. Transcriptional profiling of the ΔphaC1 mutant reveals that the loss of PHB synthesis affects the expression of many genes, including approximately 30% of the Fnr regulon. |
| Databáze: | OpenAIRE |
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